12 myristate 13 acetate

Lymphoprep™ gradient was purchased from Progen (Heidelberg, Germany). RPMI 1640 medium, fetal calf serum, trypsin 10x (25 mg/mL), trypsin-EDTA 10x (5 mg/mL trypsin and 2.2 mg/mL EDTA), and phosphate buffered saline (PBS, without Ca and Mg) were from PAA Laboratories Gmbh (Coelbe, Germany). L-Glutamine, penicillin, and streptomycin were from Invitrogen (Karlsruhe, Germany). Camptothecin was from Tocris (Eching, Germany); Triton-X 100, milk powder, and N,N,N′,N′-tetramethyl-1-,2-diaminomethane (Temed) were from Carl Roth (Karlsruhe, Germany); DMSO, lipopolysaccharide (LPS), phorbol, 12-myristate 13-acetate, CFSE, ammonium persulfate, bovine serum albumin, ethanol absolute, hydrochloric acid (37%), leupeptin hemisulfate, p-coumaric acid, pepstatin A, Ponceau S, trypan blue, Tween 20, and ionomycin were from Sigma-Aldrich (Taufkirchen, Germany).
Antibodies against p-38 (Thr180/Tyr182), p-ERK1/2 (Thr202/Tyr204), p-JNK, and p-c-Jun (Ser73) and the horseradish peroxidase- (HRP-) labelled secondary antibodies, anti-mouse and anti-rabbit, were from Cell Signaling Technology (Boston, USA); anti-human COX-2 was from R&D Systems (Wiesbaden, Germany); anti-human COX-1 and anti-human 5-LOX (mouse monoclonal, clone 33) were from Santa Cruz Biotechnology (Heidelberg, Germany); and mAb against β-actin was from Sigma-Aldrich (Taufkirchen, Germany).

RPMI 1640 Medium, L-glutamine, alantolactone ≥98% (HPLC), clarithromycin ≥95% (HPLC), Lysostaphin from Staphylococcus staphylolyticus, 12-myristate 13-acetate (PMA), DAPI solution were bought from Sigma-Aldrich Chemie GmbH (Steinheim, Germany). Fetal calf serum (FCS) and Phosphate buffered saline (PBS) were obtained from Gibco (Grand Island, NY, USA) and penicillin–streptomycin from PAA Laboratories (Pasching, Austria). Accutase™ Cell detachment solution from BD Biosciences (San Jose, CA, USA). Cellular ROS/Superoxide Detection Assays were obtained from Abcam (Cambridge, UK). S. aureus 25923 was bought from ATCC (Virginia, USA). Alexa 647 carboxylic acid, Alexa Fluor 633 phalloidin and TMR- and biotin-conjugated dextran were obtained from Thermo Fisher (Massachusetts, USA). NF-κB p65 Transcription Factor Assay kit was bought from Active Motif (CA, USA). Enzyme-linked immunosorbent assays (ELISA) were obtained from BD Biosciences (San Jose, USA).