31000v2 dapi filter set

Each dispersed cell population on an ITO-coated glass slide was imaged using an Axio Imager M2 (Carl Zeiss, Oberkochen, Germany) in fluorescence and bright-field modes. An X-CITE 120 mercury lamp (Lumen Dynamics, Mississauga, Canada) and a 31000v2 DAPI filter set (Chroma Technology, Irvine, CA) were employed for fluorescence imaging. Because the ITO glass slides are transparent and conductive, they are compatible for both MALDI MS and SIMS single cell profiling experiments. A 10× objective was used to obtain a mosaic image of the targeted surface with 13% overlap between neighboring images. Images were taken using an Axio-Cam 503 Mono camera (Carl Zeiss) with a resolution of 1936 × 1460 pixels. All mosaic optical images were stitched with a minimum overlap of 5% and maximum shift of 10%. The stitched image was loaded into lab-built software^{32 (link)} for either manual or automatic cell finding. The fiducial marks were used to register the image coordinates to the x,y translation stage coordinate of the mass spectrometers. Based on the registration, the cell coordinates were saved in either a pattern coordinate file-format readable by the oMALDI server for SIMS experiments or a custom geometry file for the MALDI-MS FlexControl software for mass spectral acquisition.