Anti mouse monoclonal antibodies

Manufactured by Thermo Fisher Scientific

Sourced in United States

Anti-mouse monoclonal antibodies are laboratory reagents used in various immunological and biochemical applications. They are designed to specifically bind to and detect mouse-derived proteins or other target molecules. These antibodies can be utilized in techniques such as Western blotting, immunohistochemistry, and ELISA to identify and quantify the presence of mouse-related analytes in samples.

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Lab products found in correlation

Cellquest software, by BD (2 mentions) Cellquest pro software, by BD (1 mentions) Facscalibur, by BD (1 mentions) Fixation permeabilization buffer, by Thermo Fisher Scientific (1 mentions) Facs caliber, by BD (1 mentions) Facscalibur flow cytometer, by BD (1 mentions)

Close spelling variants of this product

Mouse anti-6xHis-tag monoclonal antibodies Fluorochrome-conjugated anti-mouse monoclonal antibodies (Abs) Monoclonal anti-mouse CD3 (Pe-Cy5-labeled) and CD4 (FITC-labeled) antibodies Mouse monoclonal antibodies Anti-mouse antibodies

4 protocols using anti mouse monoclonal antibodies

Flow Cytometry Analysis of Immune Cells

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Flow cytometry was performed according to standard procedures. Data acquisition was performed on a FACSCalibur (BD), and data were analyzed using the CellQuest Pro software (BD).
The following listed fluorescent-labeled anti-mouse monoclonal antibodies (and clone names) all purchased from eBioscience (San Diego, CA): B220 (RA3-6B2), CD4 (GK1.5), DO11.10 TCR (KJ1-26), CD25 (PC61.5), ICOS (C398.4 A), PD1 (J43), CTLA4 (UC10-4B9), Helios (22F6), Foxp3 (FJK-16s), LAG3 (C9B7W), c-Maf (sym0F1), IL-4 (11B11), IL-10 (JES5-16E3) and IFN-γ (XMG1.2).

Chien C.H., Yu H.C., Chen S.Y, & Chiang B.L. (2017). Characterization of c-Maf+Foxp3− Regulatory T Cells Induced by Repeated Stimulation of Antigen-Presenting B Cells. Scientific Reports, 7, 46348.

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Quantifying Regulatory T Cells in Rats

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Blood samples of four rats were collected at T₀, T₁, T₂ and T₃ for cell population analysis by flow cytometry. Venous blood (approximately 50 μl) was harvested from the eyes of anesthetized rats and collected in heparinized tubes. All blood samples were washed once in phosphate-buffered saline (PBS) containing 1 % fetal calf serum (FCS) and stained with the following combination of fluorescently labeled anti-mouse monoclonal antibodies (eBioscience, San Diego, CA, USA): anti-CD4 and anti-CD25 for 15 min. After surface staining, the samples were permeabilized by incubation with the fixation/permeabilization buffer (eBioscience, San Diego, CA, USA) and incubated with anti-Foxp3 monoclonal antibody (mAbs) for 30 min. After incubation with anti-Foxp3, cells were analyzed by flow cytometry using a Becton Dickinson (BD) FACS Caliber and Cell Quest Software (BD Biosciences, San Jose, CA, USA). Isotype-matched antibodies were used as controls for all samples.

Li Q., Yu P., Zeng Q., Luo B., Cai S., Hui K., Yu G., Zhu C., Chen X., Duan M, & Sun X. (2016). Neuroprotective Effect of Hydrogen-Rich Saline in Global Cerebral Ischemia/Reperfusion Rats: Up-Regulated Tregs and Down-Regulated miR-21, miR-210 and NF-κB Expression. Neurochemical Research, 41(10), 2655-2665.

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Splenic T-cell Immunophenotyping

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Single-cell suspensions were prepared from the spleens of the experimental mice. Surface staining was performed using the following anti-mouse monoclonal antibodies from eBiosciences (San Diego, CA) and BioLegend (San Diego, CA): fluorescein isothiocyanateeanti-ICOS, phycoerythrinconjugated anti-CD4, and phycoerythrin-Cy5econjugated anti-CXCR5.

Ma J., Feng D., Wei Y., Tian J., Tang X., Rui K., Lu L., Xu H, & Wang S. (2016). Blockade of Glucocorticoid-Induced Tumor Necrosis Factor-Receptor-Related Protein Signaling Ameliorates Murine Collagen-Induced Arthritis by Modulating Follicular Helper T Cells. The American journal of pathology, 186(6).

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Antigen Marker Expression Analysis by Flow Cytometry

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The surface expression of antigen markers was performed by flow cytometry. DCs were collected and resuspended in PBS at a concentration of 2×10 5 /ml. Cells were incubated with the following anti-mouse monoclonal antibodies (eBioscience): FITC-conjugated anti-CD40, anti-CD80 and anti-MHCII for 30 min at room temperature in the dark. Appropriate isotype-matched immunoglobulins were used as negative control. Then cells were analyzed on a FACSCalibur flow cytometer with CellQuest software (Becton Dickinson, San Jose, CA, USA). Results were expressed as the percentages of positive cells calculated as specific antibody minus the value obtained from the isotype control.

Meng Y., Chen C., Liu Y., Tian C, & Li H.H. (2017). Angiotensin II Regulates Dendritic Cells through Activation of NF-κB /p65, ERK1/2 and STAT1 Pathways. Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, 42(4).

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