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Bromo 2 deoxy uridine brdu labeling and detection kit 1

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The Bromo-2'-deoxy-uridine (BrdU) Labeling and Detection Kit is a laboratory tool used to detect and quantify cell proliferation. It provides a method for labeling and detecting cells that have incorporated BrdU, a synthetic thymidine analog, during DNA synthesis. The kit includes reagents for BrdU labeling, detection, and visualization, allowing researchers to assess cell proliferation in various experimental settings.

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BrdU labeling and detection kit (40 citations) BrdU Labeling and Detection Kit I (18 citations) BrdU labeling kit (15 citations) BrdU Labeling and Detection Kit II (10 citations) BrdU detection kit (6 citations) BrdU Labeling Detection Kit II (2 citations) Detection Kit II (2 citations)

2 protocols using bromo 2 deoxy uridine brdu labeling and detection kit 1

1

Cell Proliferation Assay with BrdU

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Cell proliferation was evaluated by Bromo-2′-deoxy-uridine (BrdU) Labeling and Detection Kit I (Roche). One hour before the end of the experiment, rCPCs were incubated with BrdU (10 μM). The cells were fixed and stained as suggested by manufacturer's instructions. Cell proliferation was determined by the percentage of BrdU-positive cells.
Piegari E., Russo R., Cappetta D., Esposito G., Urbanek K., Dell'Aversana C., Altucci L., Berrino L., Rossi F, & De Angelis A. (2016). MicroRNA-34a regulates doxorubicin-induced cardiotoxicity in rat. Oncotarget, 7(38), 62312-62326.
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2

Cell Viability and Proliferation Assays

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Cell viability was evaluated using MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) tetrazolium-Sigma-Aldrich®) assay according with manufacturer recommendation. Absorbance was immediately measured at 570 nm in microplate reader (Bio-Rad 2550). Results were expressed as percentage of undifferentiated group. Cell proliferation was monitored using bromo-2′-deoxy-uridine (BrdU) labeling and detection kit I (Roche Diagnostics) after 3, 21 and 27 days, according to manufacturer recommendation. DAPI staining was performed for cell counting at a 1:2000 dilution, for 15 min at room temperature. Positive cells were quantified using the immunofluorescence microscopy (ImageXpress) with an excitation wavelength from 450 to 500 nm. Results were expressed as percentage of BrdU-positive cells related to total number of cells.
de Paula G.S., Wilieman M., Silva K.R., Baptista L.S., Boudina S., de Souza L.L., Bento-Bernardes T., Asensi K.D., dos Santos Goldenberg R.C, & Pazos-Moura C.C. (2019). Neuromedin B receptor disruption impairs adipogenesis in mice and 3T3-L1 cells. Journal of molecular endocrinology, 63(1), 93-102.
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