Anti α tubulin

The following primary antibodies were used for western blotting or immunofluorescence staining: anti-H3T3ph (Abcam, ab130940), anti-H3S10ph (Abcam, ab14955), anti-GAPDH (Proteintech, HRP-60004), anti-caspase-3 (Cell Signaling Technology, 9662S), anti-cleaved caspase-3 (Cell Signaling Technology, 9664S), anti-α-tubulin (Active motif, 39527), anti-Nanog (Abcam, ab80892), anti-Sox2 (Abcam, ab79351), anti-Oct4 (Abcam, ab27985), anti-Klf4 (Abcam, ab129473), anti-Wnt5a (Abcam, ab235966), and anti-Pax2 (Cell Signaling Technology, 9666).

Antibodies against the N-terminal domain (anti-dCTCF_N) of dCTCF (amino acids 1–287) and amino acids 657–818 of the C-terminal domain (anti-dCTCF_C) were produced in rabbits and purified by affinity purification on Aminolink resin (Thermo Fisher Scientific, USA) according to standard protocols. These antibodies were characterized in a previous study [34 (link)]. The other antibodies used were as follows: mouse monoclonal anti-HA antibodies, clone HA-7 (#H3663, Sigma, USA); mouse monoclonal anti-FLAG antibodies, clone M2 (F1804, Sigma, USA); mouse monoclonal anti-lamin Dm0, clone ADL84.12 (#ADL84.12, DSHB, USA); rat anti-CP190; anti-histone H3 (#39163, Active Motif, USA); anti-α-tubulin (#39527, Active Motif, USA).