All DLBCL samples and clinicopathological data were obtained from the Sun Yat-sen University Cancer Center between 2006 and 2013. All patients or guardians provided written consent for the use of their data. The procedures of this study were approved by the Ethics Committee of Sun Yat-sen University Cancer Center. The wild-type TP53 human DLBCL cell lines OCI-Ly3 and OCI-Ly10 and mutated-type TP53 human DLBCL cells SU-DHL-6 and SU-DHL-10 were purchased from the Type Culture Collection of the Chinese Academy of Sciences (Shanghai, China). OCI-Ly3, OCI-Ly10, SU-DHL-6, and SU-DHL-10 cell lines were cultured in RPMI 1640 medium (Gibco, Thermo Fisher Scientific, USA) containing 10% fetal bovine serum (FBS, HyClone, USA) and 1% penicillin-streptomycin solution (Gibco, 15140-122, Life Technologies, USA). All cells were cultured at 37 °C in a humidified atmosphere containing 5% CO2. Peripheral blood mononuclear cells (PBMCs) and normal tonsil tissues were used as a control in this study.
Oci ly10
Manufactured by Thermo Fisher Scientific
Sourced in United States
The OCI-Ly10 is a piece of laboratory equipment designed for cell culture applications. It serves as an incubator that provides a controlled environment for cell growth and maintenance. The core function of the OCI-Ly10 is to maintain precise temperature, humidity, and atmospheric conditions necessary for the optimal growth and preservation of cell lines.
Automatically generated - may contain errors
Lab products found in correlation
Rpmi 1640 medium, by Thermo Fisher Scientific (2 mentions)
Penicillin streptomycin solution, by Thermo Fisher Scientific (1 mentions)
Oci ly3, by Thermo Fisher Scientific (1 mentions)
Su dhl 6, by Thermo Fisher Scientific (1 mentions)
Su dhl 10, by Thermo Fisher Scientific (1 mentions)
Oci ly10, by National Collection of Authenticated Cell Cultures (1 mentions)
Sudhl 10, by National Collection of Authenticated Cell Cultures (1 mentions)
Oci ly3, by National Collection of Authenticated Cell Cultures (1 mentions)
Sudhl 6, by National Collection of Authenticated Cell Cultures (1 mentions)
Oci ly19, by Thermo Fisher Scientific (1 mentions)
Su dhl 4, by Shanghai Cell Bank (1 mentions)
U2932, by Thermo Fisher Scientific (1 mentions)
Sudhl4, by Thermo Fisher Scientific (1 mentions)
Iscove s modified dulbecco s medium imdm, by Thermo Fisher Scientific (1 mentions)
Iscove modified dulbecco media (imdm), by Thermo Fisher Scientific (1 mentions)
2 protocols using oci ly10
1
DLBCL Cell Line Characterization
2
Culturing Diverse B-Cell Lymphoma Lines
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Human normal B-cell immortalized cell line (HMy2.CIR), DLBCL cell line, germinal central B-cell (GCB)-like OCI-Ly19 and SU-DHL-4, and ABC-like OCI-LY10 and U2932 were purchased from Shanghai Cell Bank of the Chinese Academy of Sciences. HMy2.CIR was cultured in Iscove’s modified dulbecco’s medium (IMDM) (Gibco, Carlsbad, CA, USA) containing 10% fetal bovine serum (FBS), and 1% penicillin–streptomycin (P/S). U2932 and SU-DHL-4 were cultured in RPMI 1640 medium (Gibco) containing 10% FBS and 1% P/S. OCI-LY10 and OCI-Ly19 were cultured in IMDM (Gibco) containing 20% FBS and 1% P/S. All cells were maintained in a humid incubator with 5% CO2 at 37°C.
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