RNA was extracted following the Direct-zol RNA MicroPrep kit (Zymo Research) manufacturer’s instructions. RNA was treated with DNAse I (Ambion) and enriched for mRNA using Dynabeads mRNA DIRECT Purification Kit (Thermo Fisher). cDNA was generated using a custom scaled-down modification of the SMART-seq protocol [36 (link)]. cDNA was synthesized from RNA input using Maxima H Minus Reverse Transcriptase (Thermo Fisher Scientific). It was then amplified using Kapa HiFi 2X Ready Mix (Kapa Biosystems) and cleaned using 0.9X Ampure beads (Beckman Coulter). Finally, cleaned cDNA samples were tagmented and indexed using the Nextera XT DNA Library Prep Kit (Illumina). Library size and tagmentation were confirmed using the TapeStation HS D1000 kit (Agilent). Libraries were pooled in equal molarity and sequenced with the NextSeq 550 High-Output kit (Illumina) with paired-end 75 bp reads.
Tapestation hs d1000 kit
Manufactured by Agilent Technologies
The TapeStation HS D1000 kit is a lab equipment product designed for the analysis of high molecular weight DNA samples. It provides automated and sensitive size analysis of DNA fragments up to 12,000 base pairs in length.
Automatically generated - may contain errors
Lab products found in correlation
Dnase 1, by Thermo Fisher Scientific (2 mentions)
Direct zol rna microprep kit, by Zymo Research (2 mentions)
Maxima h minus reverse transcriptase, by Thermo Fisher Scientific (2 mentions)
Kapa hifi 2x readymix, by Roche (2 mentions)
Ampure bead, by Beckman Coulter (2 mentions)
Nextera xt dna library prep kit, by Illumina (2 mentions)
Dynabeads mrna direct purification kit, by Thermo Fisher Scientific (2 mentions)
Nextseq 550 high output kit, by Illumina (2 mentions)
2 protocols using tapestation hs d1000 kit
1
Single-Cell RNA Sequencing Pipeline
2
Single-Cell RNA-seq Library Preparation
Check if the same lab product or an alternative is used in the 5 most similar protocols
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RNA was extracted following the Direct-zol RNA MicroPrep kit (Zymo Research) manufacturer's instructions. RNA was treated with DNAse I (Ambion) and enriched for mRNA using Dynabeads mRNA DIRECT Purification Kit (Thermo Fisher). cDNA was generated using a custom scaled-down modification of the SMART-seq protocol (Picelli et al., 2014) . cDNA was synthesized from RNA input using Maxima H Minus Reverse Transcriptase (Thermo Fisher Scientific). It was then amplified using Kapa HiFi 2X Ready Mix (Kapa Biosystems) and cleaned using 0.9X Ampure beads (Beckman Coulter). Finally, cleaned cDNA samples were tagmented and indexed using the Nextera XT DNA Library Prep Kit (Illumina). Library size and tagmentation were confirmed using the TapeStation HS D1000 kit (Agilent). Libraries were pooled in equal molarity and sequenced with the NextSeq 550 High-Output kit (Illumina) with paired-end 75 bp reads.
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