hMSCs were isolated from the bone marrow aspirate with full ethical approval (Ethics Committee of University of Freiburg Medical Centre-EK-Freiburg: 135/14), as described in a previous study [39 (link)]. hMSCs were subcultured on α-MEM (Gibco) supplemented with 10% v/v Sera Plus bovine serum (PAN Biotech), 100 U/ml of penicillin, 100 μg/ml of streptomycin (Gibco), and 5 ng/ml of basic fibroblast growth factor (Fitzgerald Industries International) in a humidified atmosphere of 5% CO2, with the medium being changed every second day.
Sera plus bovine serum
Manufactured by PAN Biotech
Sourced in Germany
Sera Plus is a bovine serum produced by PAN Biotech. It is a cell culture supplement that provides essential nutrients and growth factors to support the growth and proliferation of cells in vitro.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using sera plus bovine serum
1
Isolation and Culture of hMSCs
2
Expansion of Human Bone Marrow-Derived Mesenchymal Stem Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Human BM-MSCs are isolated from bone marrow aspirates and cryopreserved as previously described [29 (link)]. Upon thawing, BM-MSCs are seeded at a density of 3 × 103 cells/cm2 in T300 flasks for culture expansion and cells from all donors are used up to passage 5. The expansion medium consists of α-MEM (Gibco, Carlsbad, CA, USA) supplemented with 10% (v/v) Sera Plus bovine serum (PAN-Biotech, Aidenbach, Germany), 100 U/mL Penicillin, 100 µg/mL Streptomycin (PEN/STREP) (Gibco) and 5 ng/mL basic fibroblast growth factor (Fitzgerald Industries, North Acton, MA, USA). Cells are cultured under standard conditions of 37 °C with 5% CO2 and 90% humidity. Donor details of the BM-MSCs used in this study are as follows: Donor A—48-year old male, spine vertebral body aspirate; Donor B—57-year old female, spine vertebral body aspirate; Donor C—73-year old female, spine vertebral body aspirate; and Donor D—53-year old male, femoral cancellous bone.
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