Dp215

Manufactured by Tiangen Biotech

Sourced in China

The DP215 is a high-performance digital pipette designed for accurate and precise liquid handling. It features an ergonomic design, adjustable volume range, and electronic controls for improved efficiency and reproducibility in laboratory environments.

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Lab products found in correlation

Methyl primer express v1, by Thermo Fisher Scientific (2 mentions) Em101, by Tiangen Biotech (2 mentions) Dna bisulfite conversion kit, by Tiangen Biotech (1 mentions) Dna extraction kit dp304, by Tiangen Biotech (1 mentions) Dnaiso reagent, by Takara Bio (1 mentions)

5 protocols using dp215

Nrf2 Promoter DNA Methylation Analysis

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DNA methylation was examined by bisulfite conversion of extracted genomic DNA with a bisulfite conversion kit (DP215, TIANGEN). The Nrf2 promoter was amplified by polymerase chain reaction (PCR) using a methylation-specific PCR kit (EM101, TIANGEN). Primer sequences were designed using Methyl Primer Express v1.0 (Applied Biosystems), and Sanger sequencing was performed on the PCR products. Primers were designed as follows: Nrf2-bisulfite sequencing PCR (BSP) forward: 5´-GTTTGTTGGGATTTTAGTTGGTAGTT-3´; Nrf2-BSP reverse: 5´-CCAAATCCATCATAATAAACTATAAACC-3´.

Liu K, & Xu Y. (2023). Downregulation of TET2 Contributes to Nasal Polypogenesis Through Hypoxia-Inducible Factor 1α-Mediated Epithelial-to-Mesenchymal Transition. Clinical and Experimental Otorhinolaryngology, 17(1), 64-77.

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DNA Methylation Analysis of Prostate Cell Lines

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DNA from RWPE-1 cells, PC3 cells, DU145 cells, and LNCaP cells were extracted using DNA extraction kit (DP304) (TIANGEN, Beijing, China). The sodium bisulfite DNA treatments were conducted using a DNA bisulfite conversion kit (TIANGEN, DP215) according to the manufacturer's instructions. PCR amplifications were performed using Phanta® UC Super-Fidelity DNA Polymerase (Vazyme, P507-01) according to the manufacturer's instructions. The PCR primers for methylation-specific PCR were listed in Table S6.

Chen Y., Pan C., Wang X., Xu D., Ma Y., Hu J., Chen P., Xiang Z., Rao Q, & Han X. (2021). Silencing of METTL3 effectively hinders invasion and metastasis of prostate cancer cells. Theranostics, 11(16), 7640-7657.

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DNA Methylation Analysis of IFN-γ Promoter

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DNA methylation was examined by bisulfite conversion of extracted genomic DNA with a bisulfite conversion kit (#DP215, TIANGEN, China). The IFN-γ promoter was amplified by PCR using a methylation-specific PCR kit (#EM101, TIANGEN, China). Primer sequences were designed using Methyl Primer Express v1.0 (Applied Biosystems, USA), and Sanger sequencing was performed on the PCR products. Five individual PCR products were sequenced for each sample. Primers we designed are as follows: IFN-γ-BSP forward: 5’- TTTAGTATTTTGGGAGGTTAAGG-3’; reverse: 5’-ATCACCCAAACTAAAATACAATAAC-3’.

Wang H., Xie L., Song X., Wang J., Li X., Lin Z., Su T., Liang B, & Huang D. (2022). Purine-Induced IFN-γ Promotes Uric Acid Production by Upregulating Xanthine Oxidoreductase Expression. Frontiers in Immunology, 13, 773001.

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Methylation Analysis of Mouse ABCG1

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Genomic DNA was extracted from mouse BMDM using the DNAiso reagent (9770Q, Takara, Kusatsu, Shiga, Japan). A Bisulfte Conversion Reaction (DP215, TIANGEN, Beijing, China) was then conducted to convert unmethylated C to U; whereas methylated C remains unchanged. Bisulfite-treated DNA was then used as a template for methylation specific PCR according to the manufacturer’s instructions (R100A, Takara, Kusatsu, Shiga, Japan). The MS-qPCR primers (M primer, U primer) were designed using MethPrimer 2.0 using a template 1000 bp upstream and downstream of the mouse ABCG1 transcription start site (TSS).

Zhang M., Zeng X., Yang Q., Xu J., Liu Z., Zhou Y., Cao Y., Zhang X., An X., Xu Y., Huang L., Han Z., Wang T., Wu C., Fulton D.J., Weintraub N.L., Hong M, & Huo Y. (2018). Ablation of myeloid adenosine kinase epigenetically suppresses atherosclerosis in apolipoprotein E-deficient mice. Arteriosclerosis, thrombosis, and vascular biology, 38(12), 2780-2792.

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Bisulfite Sequencing Protocol for DNA Methylation

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Genomics was extracted from cells, and DNA was treated with a bisulfite kit (DP215 TIANGEN) Then carried out DNA amplification. The fragments were recovered and connected with the carrier. Carrier was used to transformant competent cells. After 12 h of culture, 10 colonies were selected for sequencing. Used the online website (http://quma.cdb.riken.jp/top/index.html. accessed on 15 May 2019) to make a pie chart. PCR Primer sequences see Supplementary Table S4.

Zhang C., Zuo Q., Gao X., Hu C., Zhou S., Chen C., Zou Y., Zhao J., Zhang Y, & Li B. (2021). H3K4me2 Promotes the Activation of lncCPSET1 by Jun in the Chicken PGC Formation. Animals : an Open Access Journal from MDPI, 11(6), 1572.

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