Nuclear proteins were extracted from proliferating cells in acid extraction buffer (0.2N HCL), following the extraction of cytoplasmic proteins with TNE buffer: 150 mM NaCl, 10 mM Tris pH 7.4, 1% Triton X-100, 5 mM EDTA, 1% NP40, 1 μM DTT, and proteinase (Roche) plus phosphatase (Sigma) inhibitor cocktails. Protein extracts were resolved by SDS-PAGE and transferred to poly(vinylidene difluoride) (PVDF) membranes. After probing with primary antibodies, the membranes were incubated with horseradish peroxidase-conjugated secondary antibody, and signals visualized by ECL (GE Healthcare). Antibodies specific for total histone H3 (96C10, 1:1,000), H3K27me3 (C36B11, 1:1,000), H3K27me2 (D18C8, 1:1,000), H3K27me1 (#7693 1:1,000), and EZH2 (D2C9, 1:1,000), were obtained from Cell Signaling Technologies. Histone H3.3 antibody (ab97968, 1:1,000) was obtained from Abcam, and histone K27M mutant antibody (ABE419, 1:1,000) was from EMD Millipore.
Antibodies specific for total histone h3 96c10
Manufactured by Cell Signaling Technology
Antibodies specific for total histone H3 (96C10) are laboratory reagents used to detect and quantify the presence of histone H3 in various biological samples. These antibodies recognize the total pool of histone H3 proteins, regardless of any post-translational modifications. They serve as a tool for researchers studying chromatin structure, gene regulation, and epigenetic mechanisms.
Automatically generated - may contain errors
Lab products found in correlation
H3k27me2 d18c8, by Cell Signaling Technology (2 mentions)
Histone h3.3 antibody ab97968, by Abcam (2 mentions)
Histone k27m mutant antibody abe419, by Merck Group (2 mentions)
H3k27me3 c36b11, by Cell Signaling Technology (2 mentions)
Enhanced chemi luminescence (ecl), by GE Healthcare (2 mentions)
Ezh2 d2c9, by Cell Signaling Technology (2 mentions)
H3k27me1, by Cell Signaling Technology (2 mentions)
2 protocols using antibodies specific for total histone h3 96c10
1
Histone Modification Detection Assay
2
Histone Modification Detection Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Nuclear proteins were extracted from proliferating cells in acid extraction buffer (0.2N HCL), following the extraction of cytoplasmic proteins with TNE buffer: 150 mM NaCl, 10 mM Tris pH 7.4, 1% Triton X-100, 5 mM EDTA, 1% NP40, 1 μM DTT, and proteinase (Roche) plus phosphatase (Sigma) inhibitor cocktails. Protein extracts were resolved by SDS-PAGE and transferred to poly(vinylidene difluoride) (PVDF) membranes. After probing with primary antibodies, the membranes were incubated with horseradish peroxidase-conjugated secondary antibody, and signals visualized by ECL (GE Healthcare). Antibodies specific for total histone H3 (96C10, 1:1,000), H3K27me3 (C36B11, 1:1,000), H3K27me2 (D18C8, 1:1,000), H3K27me1 (#7693 1:1,000), and EZH2 (D2C9, 1:1,000), were obtained from Cell Signaling Technologies. Histone H3.3 antibody (ab97968, 1:1,000) was obtained from Abcam, and histone K27M mutant antibody (ABE419, 1:1,000) was from EMD Millipore.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!