Mouse lung tissue was paraffin-embedded and cut into 4-µm sections. Sections were blocked with 5% serum, followed by overnight incubation with a primary goat anti-mouse IL-33 antibody (R&D Systems) at 4°C. Then sections were incubated with a secondary donkey anti-goat IgG antibody (R&D Systems) and staining was visualised with 3,3′-diaminobenzidine (Vector Laboratories, BioNordika, Stockholm, Sweden) and background stained with haematoxylin. Stained sections were scanned (Aperio; Leica Microsystems, Bromma, Sweden).
Primary goat anti mouse il 33 antibody
Manufactured by R&D Systems
Sourced in United Kingdom
The Primary goat anti-mouse IL-33 antibody is a laboratory reagent used for the detection and quantification of mouse interleukin-33 (IL-33) in various biological samples. This antibody specifically binds to the IL-33 protein, allowing researchers to study its expression and function in mouse models and research applications.
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Lab products found in correlation
2 protocols using primary goat anti mouse il 33 antibody
1
Immunohistochemical Analysis of IL-33 in Mouse Lung
2
Visualizing Lung IL-33 Expression
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Paraffin embedded lung tissues were sectioned 4 µm thick. Immunohistochemistry was used to visualise lung tissue IL-33 expression in paraffin embedded sections. The sections were first blocked with 5 % serum, following overnight incubation at 4 °C with primary goat anti-mouse IL-33 antibody (R&D Systems, UK). After rinsing the sections and incubating with secondary anti-goat IgG antibody (R&D Systems, UK), the staining was visualised with 3,3′-diaminobenzidine (DAB, Vectastain, Vector Laboratories, USA) and background stained with haematoxylin. All IL-33 stained sections were scanned (Aperio Technologies, USA) with software Aperio ScanScopeTM and representative photos were chosen and presented for each stimulus group in the study.
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