Fasting (16 h) blood glucose levels were measured from tail blood using the Contour NEXT Glucometer (Bayer, Germany). Plasma samples were obtained from tail blood after fasting or following glucose administration using blood collection tubes treated with EDTA (Sarstedt, Germany). Plasma glucagon and insulin levels were assessed using mouse ELISA assays (Mercodia, Sweden and Crystal Chem, USA, respectively). Plasma l -amino acids were measured using l -Amino Acid Quantitation Kit (Sigma, USA). See ESM Table 2 for further details.
Mouse elisa assays
Manufactured by Mercodia
Sourced in Sweden
Mouse ELISA assays are laboratory equipment used to detect and quantify the presence of specific proteins or other analytes in mouse biological samples. The core function of these assays is to provide a sensitive and accurate measurement of the target analyte through an enzyme-linked immunosorbent assay (ELISA) technique.
Automatically generated - may contain errors
2 protocols using mouse elisa assays
1
Plasma metabolite profiling of fasted mice
2
Plasma metabolite profiling of fasted mice
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Fasting (16 h) blood glucose levels were measured from tail blood using the Contour NEXT Glucometer (Bayer, Germany). Plasma samples were obtained from tail blood after fasting or following glucose administration using blood collection tubes treated with EDTA (Sarstedt, Germany). Plasma glucagon and insulin levels were assessed using mouse ELISA assays (Mercodia, Sweden and Crystal Chem, USA, respectively). Plasma l -amino acids were measured using l -Amino Acid Quantitation Kit (Sigma, USA). See ESM Table 2 for further details.
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