Eagle sminimal essential medium

EMT6/AR1 cells were procured from Sigma and were cultured in Eagle’s
minimal essential medium (MEM) (HiMedia). MCF-7, HeLa, A549, B16F10,
and L929 cells were obtained from National Centre for Cell Science,
Pune. The cell lines were authenticated using STR analysis and they
were tested free from mycoplasma. MCF-7, HeLa, B16F10, and L929
cells were cultured in Dulbecco’s modified eagle’s medium
(DMEM) (HiMedia). A549 cells were cultured in F-12k medium
(Kaighn’s Modification of Ham’s F-12 Medium) (HiMedia).
MCF10A cells were cultured in 1:1 mixture of DMEM/F12 medium
supplemented with 0.5 μg/mL hydrocortisone, 20 ng/mL
EGF, and 10 μg/mL insulin. The cells were maintained as described
earlier.^{62 (link)}