DSC was performed using a DSC 250 instrument (TA Instruments). Oligonucleotides were first dissolved in PBS with 0.1% (v/v) Tween 20 to 500 μM, and their concentrations were measured with NanoDrop. Next, the relevant oligos were mixed to create 100 μM duplex. Sample solutions were pre-annealed by heating at 95°C for 3 min and cooling down to 25°C at the rate of −1°C per 10 sec. Sample and buffer solutions were then degassed by ultrasonication for 10 min using Bransonic 1800 (Branson). Twenty microliters of each solution were placed in a Tzero aluminium pan and covered with a Tzero aluminium hermatic lid (TA Instruments). Starting from incubating at 40°C for 5 min, the samples were heated to 110°C at the rate of 5°C/min during DSC.
Dsc 250 instrument
Manufactured by TA Instruments
Sourced in United States
The DSC 250 is a differential scanning calorimetry (DSC) instrument manufactured by TA Instruments. The core function of the DSC 250 is to measure the heat flow and temperature changes associated with material transitions and transformations as a function of time and temperature.
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Lab products found in correlation
2 protocols using dsc 250 instrument
1
Oligonucleotide Duplex Thermal Analysis
2
Thermal Analysis of APR and SNEDDS
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DSC analysis was performed with a TA Instruments DSC 250 instrument (New Castle, DE, USA). A 5 mg sample was weighed and sealed in hermetic aluminum pans (Tzero Hermetic Pan and Lid). A hermetically sealed empty aluminum pan was used as a reference. The samples were heated at a rate of 10 °C/minute between 30 and 350 °C. Nitrogen gas was used as the inert gas at a flow rate of 50 mL/min. Powder APR and SNEDDS formulation thermograms were analyzed with the TRIOS program.
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