Clarity chemiluminescent kit

Manufactured by Bio-Rad

The Clarity Chemiluminescent kit is a laboratory reagent used for the detection and quantification of proteins in Western blot analysis. The kit provides a luminescent substrate that reacts with the horseradish peroxidase (HRP) conjugate, producing a luminescent signal proportional to the amount of target protein present. The kit is designed to offer a sensitive and reliable method for protein analysis in a variety of research applications.

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Lab products found in correlation

Nitrocellulose membrane, by Bio-Rad (1 mentions)

Spelling variants of this product

Clarity western chemiluminescent (ECL) substrate kit (2 citations)

2 protocols using clarity chemiluminescent kit

IDO1 Protein Expression Analysis

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Protein samples (40µg) were analyzed on 10% SDS-PAGE and transferred onto nitrocellulose membrane (Bio-Rad Laboratories). The used detection primary antibody was mouse anti‐human IDO1 (1:10000, Millipore). Immunodetection was performed by Clarity Chemiluminescent kit (Bio-Rad Laboratories).

Montanucci P., Bistoni O., Antonucci M., Pescara T., Greco A., Basta G., Bartoloni E., Gerli R, & Calafiore R. (2023). Emerging of a new CD3+CD31HCD184+ tang cell phenothype in Sjögren’s syndrome induced by microencapsulated human umbilical cord matrix-derived multipotent stromal cells. Frontiers in Immunology, 14, 1095768.

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Quantitative Western Blot Analysis

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For Western blots, 2 × 10⁶ K562 cells were treated with indicated reagents, lysed using radioimmunoprecipitation assay (RIPA) buffer and probed with indicated antibodies [31 (link)]. Twenty-five micrograms of protein from each lysate was run on a SDS/PAGE (10 %gel) followed by transfer to PVDF membrane. Membranes were incubated overnight at 4 °C with primary antibodies to either PPARα (1:250) or HIF-1α (1:250). Membranes were washed and incubated with the appropriate secondary antibodies (1:10000), followed by development using the Clarity chemiluminescent kit (Bio-Rad). The membranes were stripped and re-probed with an antibody for β-actin (1:20000), as a loading control. Quantification of images was performed using the ImageJ software suite.

Gonsalves C.S., Li C., Mpollo M.S., Pullarkat V., Malik P., Tahara S.M, & Kalra V.K. (2015). Erythropoietin-mediated expression of placenta growth factor is regulated via activation of hypoxia-inducible factor-1α and post-transcriptionally by miR-214 in sickle cell disease. The Biochemical journal, 468(3), 409-423.

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