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Anti cytokeratin 5 anti rabbit cy5

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Anti-cytokeratin-5/anti-rabbit (Cy5) is a fluorescently labeled antibody reagent. It can be used to detect and analyze cytokeratin-5 proteins in biological samples.

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Lab products found in correlation

Axiovert fluorescent microscope, by Zeiss (3 mentions) Anti cd11c, by BD (2 mentions) Anti cytokeratin 8 anti rat cy3, by BD (2 mentions)

2 protocols using anti cytokeratin 5 anti rabbit cy5

1

Immunohistochemical Profiling of Thymic Lobes

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Thymic lobes were frozen in OCT prior to cutting 10 μm sections that were fixed in 2% paraformaldehyde, blocked with PBS/5% FBS/0.05% triton and stained with combinations of anti-CD11c (FITC); anti-cytokeratin-5/anti-rabbit (Cy5); and anti-cytokeratin-8/anti-rat Cy3 (antibodies obtained from BD Biosciences; Jackson Immunoresearch Laboratories, Vector Labs). Images were acquired and tiled with a Zeiss Axiovert Fluorescent Microscope.
Moore A.J., In T.S., Trotman-Grant A., Yoganathan K., Montpellier B., Guidos C.J., Zúñiga-Pflücker J.C, & Anderson M.K. (2017). A key role for IL-7R in the generation of microenvironments required for thymic dendritic cells. Immunology and Cell Biology, 95(10), 933-942.
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2

Immunofluorescent Staining of Thymus

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Thymic lobes were frozen in optimal cutting temperature (OCT) before cutting 10 μm sections that were fixed in 2% paraformaldehyde, blocked with PBS/5% fetal bovine serum/0.05% Triton and stained with combinations of anti-CD11c (fluoroscein isothiocyanate); anti-cytokeratin-5/anti-rabbit Cy5; and anti-cytokeratin-8/anti-rat Cy3 (antibodies obtained from BD Biosciences, Jackson Immunoresearch Laboratories (West Grove, PA, USA) and Vector Labs). Images were acquired and tiled with a Zeiss Axiovert Fluorescent Microscope (Carl Zeiss Canada Ltd, North York, ON, Canada).
Moore A.J., In T.S., Trotman-Grant A., Yoganathan K., Montpellier B., Guidos C.J., Zúñiga-Pflücker J.C, & Anderson M.K. (2017). A key role for IL-7R in the generation of microenvironments required for thymic dendritic cells. Immunology and Cell Biology, 95(10), 933-942.
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