Antibody against active β catenin

Tenovin-6 (chemical structure, Fig. 1A) was purchased from Cayman Chemical (Ann Arbor, MI). Annexin V–FITC and N-acetylcysteine (NAC) were from Sigma-Aldrich (Shanghai, China). CM-H2DCF-DA and antibody against cytochrome c oxidase subunit II (COXII) were purchased from Invitrogen (Shanghai, China). Antibodies against SIRT1 (H-300), p53 (DO-7), Bax, DVL1, DVL3, β-catenin, cyclin D1 (C-20), survivin and c-Myc were from Santa Cruz Biotech. (Santa Cruz, CA). Antibody against active-β-catenin was from Millipore and the fluorescent secondary antibody was purchased from life technologies (Eugene. OR). Antibodies against PARP (clone 4C10-5), caspase-3, cytochrome c (clone 6H2.B4), XIAP, Bcl-2, phospho-GSK3β (Y216) were purchased from BD Biosciences (San Jose, CA). Antibodies against K382-acetyl-p53, DVL2, phospho-GSK3β (S9), MMP2 and MMP9 were from Cell Signaling Tech. (Beverly, MA). Anti-SIRT2 was purchased from Atlas Antibodies. Anti-mouse immunoglobulin G and anti-rabbit immunoglobulin G fluorescent-conjugated secondary antibodies were from LI-COR Biotechnology (Nebraska, USA).

GRP, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and bovine serum albumin (BSA) were purchased from Sigma. Cell culture reagents were obtained from Gibco. Antibodies against Smad2/3, p-Smad2/3, Smad4, Wnt4a, β-catenin, Dickkopf-related protein 1 (Dkk-1), E-cadherin and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) were from Santa Cruz. Antibodies against Smad7, Wnt7a, collagen 1α1, rabbit IgG, mouse IgG were from Abcam. Antibodies against Wnt-5a and α-SMA were from Novus. Antibody against active β-catenin was from Millipore. PCR reagents were purchased from Applied Biosystems. Bromodeoxyuridine (BrdU) cell proliferation assay, RNA isolation and complementary DNA (cDNA) synthesis kits were purchased from Millipore, Life Technology and Roche, respectively.