Luria Bertani (LB) media was purchased from Oxoid Limited (Hampshire, UK). Acetonitrile was purchased from Concord Technology (Minnesota, USA). Formic acid was purchased from Merck (New Jersey, USA). Skatole, S-(5′-Adenosyl)-l -methionine iodide, and 5′-deoxyadenosine were purchased from Sigma Aldrich (Saint Louis, USA). Trifluoroacetic acid and 2,3-dimethylindole were purchased from J&K (Beijing, China). Talon resin was purchased from Clonetech laboratories Inc. (California, USA). All protein purification chromatographic experiments were performed on an ÄKTA pure or ÄKTA prime plus FPLC machines equipped with appropriate columns (GE Healthcare, USA). Protein concentrations were calculated from the absorption at 280 nm measured using an Eppendorf BioPhotometer® D30. Anaerobic experiments were conducted in a Lab2000 glovebox (Etelux) under an atmosphere of N2 with less than 10 ppm O2.
5 deoxyadenosine
Manufactured by Merck Group
Sourced in United States, China
5'-deoxyadenosine is a nucleoside analog that consists of a purine base, adenine, and a sugar moiety, 5'-deoxyribose. It is a naturally occurring compound found in various organisms. As a lab equipment product, 5'-deoxyadenosine can be used for research purposes, but a detailed description of its core function is not available without extrapolation.
Automatically generated - may contain errors
Lab products found in correlation
Luria bertani media, by Thermo Fisher Scientific (1 mentions)
Appropriate columns, by GE Healthcare (1 mentions)
Formic acid, by Merck Group (1 mentions)
Skatole, by Merck Group (1 mentions)
Trifluoroacetic acid, by J&K Scientific (1 mentions)
S 5 adenosyl l methionine iodide, by Merck Group (1 mentions)
Inosine, by Merck Group (1 mentions)
Pseudouridine, by Merck Group (1 mentions)
N4 acetylcytidine, by Merck Group (1 mentions)
Creatinine, by Merck Group (1 mentions)
Milli q apparatus, by Merck Group (1 mentions)
Xanthosine, by Merck Group (1 mentions)
5 methyluridine, by Merck Group (1 mentions)
N6 methyladenosine, by Merck Group (1 mentions)
1 methyladenosine, by Merck Group (1 mentions)
3 acrylamido phenylboronic acid aapba, by Merck Group (1 mentions)
2 protocols using 5 deoxyadenosine
1
Purification of Methyltransferase Enzymes
2
Capillary-Based Silica Functionalization for Nucleoside Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Fused-silica capillary (250 μm i.d. × 360 μm o.d.) was purchased from Yongnian Optic Fiber Plant (Hebei, China). Tetramethoxysilane (TMOS) and 3-mercaptopropyltrimethoxysilane (MPTMS) were purchased from Wuhan University Silicone New Material (Wuhan, China). Azobisisobutyronitrile (AIBN) and poly(ethylene glycol) with the molecular weight of 6000 (PEG-6000) were all purchased from Shanghai Chemical Reagent Corporation (Shanghai, China). AIBN was purified by recrystallization from ethanol at 40°C. 3-acrylamidophenylboronic acid (AAPBA) and creatinine were purchased from Sigma-Aldrich (Beijing, China). Organic solvents were all of HPLC grade. The water used throughout all experiments was purified using a Milli-Q apparatus (Millipore, Bradford, USA). All other reagents were obtained from various commercial sources and were of analytical grade unless otherwise indicated.
2′-Deoxycytidine (dC), 2′-deoxyguanosine (dG), 2′-deoxyadenosine (dA), thymidine (T), cytidine (rC), guanosine (rG), adenosine (rA), uridine (rU), 1-methyladenosine, N6-methyladenosine, 5′-deoxyadenosine, inosine, xanthosine, 3-methylcytidine, N4-acetylcytidine, 5-methyluridine, 3-methyluridine, pseudouridine, double hydrogen zeatin-riboside (DHzR) were purchased from Sigma-Aldrich (Beijing, China). The standard solution of each analyte was prepared at 1.0 mg/mL in H2O and stored at −20°C.
2′-Deoxycytidine (dC), 2′-deoxyguanosine (dG), 2′-deoxyadenosine (dA), thymidine (T), cytidine (rC), guanosine (rG), adenosine (rA), uridine (rU), 1-methyladenosine, N6-methyladenosine, 5′-deoxyadenosine, inosine, xanthosine, 3-methylcytidine, N4-acetylcytidine, 5-methyluridine, 3-methyluridine, pseudouridine, double hydrogen zeatin-riboside (DHzR) were purchased from Sigma-Aldrich (Beijing, China). The standard solution of each analyte was prepared at 1.0 mg/mL in H2O and stored at −20°C.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!