The ID-NAT yield donations were tested further with the discriminatory COBAS AmpliPrep/COBAS TaqMan HBV test, COBAS AmpliPrep/COBAS TaqMan HCV test, and COBAS AmpliPrep/COBAS TaqMan HIV test (Roche Molecular Systems) on a Cobas TaqMan analyzer (Roche Diagnostics Company, Shanghai, China). The manufacturer states that the lower limit of detection for the HBV DNA assay is 20 IU/mL. Hepatitis B virus DNA of follow-up samples was tested for viral load by the same method.
Cobas ampliprep cobas taqman hiv test
Manufactured by Roche
Sourced in Germany
The COBAS AmpliPrep/COBAS TaqMan HIV test is a laboratory instrument used for the quantitative detection of HIV-1 RNA in human plasma. It is designed to measure the viral load of HIV-1 in infected individuals.
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4 protocols using cobas ampliprep cobas taqman hiv test
1
Quantifying Viral Loads with Cobas TaqMan
2
Quantitative HIV-1 RNA Measurement
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According to the manufacturer's instructions, the levels of HIV-1 RNA were measured in fresh plasma and CSF samples using quantitative PCR (COBAS AmpliPrep/COBAS TaqMan HIV test; Roche Molecular Systems, Switzerland), with a lower limit of quantification of 20 RNA copies/mL.
3
HIV Viral Load Monitoring Protocol
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HIV-1 VL was quantified by either Abbott m2000sp/Abbott m2000rt platform (Wiesbaden, Germany), Cobas TaqMan/Cobas Ampliprep HIV-test (Roche Molecular Systems, Branchburg, NJ, USA) or Aptima HIV-1 Quant assay on Panther Systems (Hologic inc., San Diego, CA, USA) at BHHRL or district-based laboratories. VF was defined as two consecutive VL greater than 400 copies/mL and virologic suppression as a viral load < 400 copies/mL as per national ART guidelines. Because of the various VL detection platforms used and changes in the national VL reporting guidelines, some of the VL data was reported as <400, <50, <40 and <25 copies/mL.
4
HIV Viral Load Testing Protocols
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HIV viral load testing from plasma specimens was performed using the Abbott m2000sp/Abbott m2000rt platform (Wiesbaden, Germany), Cobas TaqMan/Cobas Ampliprep HIV-test (Roche Molecular Systems, Branchburg, NJ, United States) at Botswana Harvard Health Partnership (BHP) according to the manufacturer’s instructions. At the time of enrolment VF was defined as two consecutive VLs greater than 400 copies/mL and virologic suppression as a viral load <400 copies/mL as per national ART guidelines. The lower limit of detection (LOD) in plasma is 40 copies/mL for this VL detection platform (See Figure 2 ).
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