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Nexcare waterproof tape

Manufactured by 3M

Nexcare waterproof tape is a medical-grade adhesive tape designed to protect wounds and incisions from exposure to water. It is made with a waterproof and breathable backing that helps maintain a sterile and moist environment for optimal healing.

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Lab products found in correlation

2 protocols using nexcare waterproof tape

1

Murine Model of MRSA Skin Infection

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All animal experiments were performed in accordance with University of Miami Animal Care and Use Committee guidelines. Animal's genotype was blinded prior to the experiment to limit bias. Our methodology was adopted from references (Cheng et al., 2009 (link); Wanke et al., 2013 (link)). In brief, all mice were shaved and tape-stripped (7 applications) with Transpore tape (3M, Minneapolis, MN, United States). This level of tape stripping did not create a wound, but was sufficient to disrupt the epidermal barrier. An inoculum of 109 MRSA strain CLP 153 in 0.02 ml of phosphate-buffered saline (PBS) or PBS control was added to ∼1 cm2 of skin and the area bandaged with plastic sheet and overwrapped with dressings of Transpore tape and Nexcare waterproof tape (3M) for 6 hr, at which time the bandage was removed. Mice were weighed daily throughout the experiment; animals were euthanized after greater than 30% weight loss.
For CFU enumeration, mice were sacrificed either 6 or 12 days after infection, cardiac puncture was performed and organs were harvested, weighed, and homogenized using a potter homogenizer in ddH2O with 0.05% Triton X-100. The homogenates were diluted and plated on TSA II plates (kanamycin and oxacillin selection). All samples were normalized based on weight.
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2

Cutaneous Abscess Induction Protocol

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The skin was shaved with an electric razor and then disinfected with iodine, washed with alcohol followed by saline, and dried with gauze. For epicutaneous inoculation (24 (link), 25 ), the skin surface was prepared by gentle tape-stripping seven times with Transpore tape (approximately 27 mm in width, 3M, Minneapolis, MN, USA). This technique was found to cause minimal damage to the epidermis and dermis (24 (link)). An inoculum of 107S. aureus CFU in 0.025 ml of saline was added to 4 mm filter paper discs placed on prepared skin of both flanks; the sites were covered with 1.0 cm2 pieces of plastic sheet and then overwrapped with dressings of Transpore tape and Nexcare waterproof tape (3M). To produce cutaneous abscesses, the same inoculum of S. aureus was injected intradermally into the flank sites.
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