STL427944 (Vitas-M Laboratory, Hong Kong), actinomycin D (ActD, MilliporeSigma, Burlington, MA, USA), cycloheximide (CHX, MilliporeSigma), bafilomycin A1 (BafA1, AdipoGen Life Sciences, San Diego, CA, USA), bortezomib (APExBIO Technology, Houston, TX, USA), MG132 (Tocris Bioscience, Minneapolis, MN, USA), docetaxel (APExBIO Technology), paclitaxel (APExBIO Technology), and 5-FU (LKT Laboratories, St Paul, MN, USA) were dissolved in DMSO. Doxycycline (LKT Laboratories), chloroquine (CQ, LKT Laboratories), and puromycin (MilliporeSigma) were dissolved in sterile water. Carboplatin (AdipoGen Life Sciences) was dissolved in sterile 5% d -glucose (MilliporeSigma) solution in water. Leptomycin B (LMB, Alfa Aesar, Haverhill, MA, USA) was dissolved in ethanol.
Bafilomycin a1 bafa1
Manufactured by Adipogen
Sourced in United States
Bafilomycin A1 (BafA1) is a macrolide antibiotic that functions as a specific inhibitor of vacuolar-type H+-ATPase (V-ATPase). V-ATPase is an enzyme responsible for acidifying cellular compartments, such as lysosomes and endosomes. By inhibiting V-ATPase, BafA1 disrupts the pH gradient within these organelles.
Automatically generated - may contain errors
Lab products found in correlation
Mg132, by Bio-Techne (1 mentions)
Docetaxel, by Apexbio (1 mentions)
Bortezomib, by Apexbio (1 mentions)
Leptomycin b, by Thermo Fisher Scientific (1 mentions)
D glucose, by Merck Group (1 mentions)
Cycloheximide (chx), by Merck Group (1 mentions)
Puromycin, by Merck Group (1 mentions)
Carboplatin, by Adipogen (1 mentions)
Paclitaxel, by Apexbio (1 mentions)
Dmem without amino acids, by Fujifilm (1 mentions)
Torin1, by Bio-Techne (1 mentions)
Lysotracker, by Thermo Fisher Scientific (1 mentions)
Rapamycin, by Merck Group (1 mentions)
Complete protease inhibitor cocktail, by Merck Group (1 mentions)
2 protocols using bafilomycin a1 bafa1
1
Comprehensive Drug Dissolution Protocol
2
Inhibiting Cellular Acidification Pathways
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Bafilomycin A1 (baf A1), a specific inhibitor of vacuolar-type H⁺-ATPase, was purchased from Adipogen. Torin 1, an mTORC1/2 inhibitor, was purchased from Tocris.
Rapamycin, an mTORC1 inhibitor, and cOmplete protease inhibitor cocktail were purchased from Sigma-Aldrich. DMEM without amino acids was purchased from Wako.
LysoTracker, a fluorescent indicator of cellular acidic fractions, was purchased from Thermo Fisher Scientific.
Rapamycin, an mTORC1 inhibitor, and cOmplete protease inhibitor cocktail were purchased from Sigma-Aldrich. DMEM without amino acids was purchased from Wako.
LysoTracker, a fluorescent indicator of cellular acidic fractions, was purchased from Thermo Fisher Scientific.
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