Analysis of organic acids was carried out by high‐performance liquid chromatography (HPLC). After adequate dilution, 1 ml of a cell‐free sample was acidified with 20 μl of 20% (v/v) H2SO4, and filtered through a 0.2 μm polypropylene filter, before analysis in a Dionex UltiMate 3000 RSLC HPLC system (Thermo Fisher Scientific, Waltham, MA, USA) connected to a Shodex RI‐101 differential refractive index detector (Showa Denko, Tokyo, Japan). The organic acids were separated using the Aminex HPX‐87H analytical column coupled to a guard column (Bio‐Rad, Hercules, CA, USA). A series of standard solutions including formic acid, acetic acid, lactic acid, butyric acid, succinic acid, propionic acid, and valeric acid were prepared and analyzed together with the samples. The temperature was set to 40℃ and the mobile phase consisted of 5 mmol H2SO4 with a flow rate of 0.5 ml/min. A small amount of acetic and succinic acid was present in the PY medium (without cells), and was subtracted when organic acids were calculated.
Dionex ultimate 3000 rslc hplc system
Manufactured by Thermo Fisher Scientific
Sourced in United States
The Dionex UltiMate 3000 RSLC HPLC system is a high-performance liquid chromatography (HPLC) instrument designed for analytical separation and quantification of chemical compounds. It features a rapid separation liquid chromatography (RSLC) technology that enables fast and efficient separations. The system is capable of operating at high pressures and flow rates to provide high-resolution separations.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using dionex ultimate 3000 rslc hplc system
1
Organic Acids Analysis by HPLC
2
Characterization of IgG Charge Variants
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Initial characterization
of IgG charge variants was performed by CEC using a BioPro IEX-SF
analytical cation exchange column (100 × 4.6 mm, 5 μm,
Cat. No. SF00S05-1046WP, YMC) on a Dionex UltiMate3000 RSLC HPLC-system
(Thermo Scientific) without carboxypeptidase B (CpB) pretreatment.
Separation was achieved with a gradient from 2 to 15% eluent B in
30 min, 15 to 100% in 0.1 and 5 min at 100% eluent B (eluent A: 20
mm BES, pH 6.8; eluent B: 20 mM BES, 488 mM NaCl, pH 6.8). A flow
rate of 0.8 mL/min, column temperature of 41 °C, and maximum
pressure of 120 bar were applied. The UV absorption was monitored
at 280 nm. Approximately, 150 μg per sample was injected for
chromatographic analysis. Data acquisition and relative quantification
by manual integration and comparison of peak areas was performed by
Chromeleon software (Thermo Scientific).
of IgG charge variants was performed by CEC using a BioPro IEX-SF
analytical cation exchange column (100 × 4.6 mm, 5 μm,
Cat. No. SF00S05-1046WP, YMC) on a Dionex UltiMate3000 RSLC HPLC-system
(Thermo Scientific) without carboxypeptidase B (CpB) pretreatment.
Separation was achieved with a gradient from 2 to 15% eluent B in
30 min, 15 to 100% in 0.1 and 5 min at 100% eluent B (eluent A: 20
mm BES, pH 6.8; eluent B: 20 mM BES, 488 mM NaCl, pH 6.8). A flow
rate of 0.8 mL/min, column temperature of 41 °C, and maximum
pressure of 120 bar were applied. The UV absorption was monitored
at 280 nm. Approximately, 150 μg per sample was injected for
chromatographic analysis. Data acquisition and relative quantification
by manual integration and comparison of peak areas was performed by
Chromeleon software (Thermo Scientific).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!