Autolab pgatat302n

Cationic CP (PMNT) was synthesized on the basis of the literature. S. oneidensis MR-1 was purchased from Marine Culture Collection of China. The liquid medium and double-distilled water (ddH₂O) were autoclaved at 120°C for 20 min. Phosphate-buffered saline (PBS) was purchased from Hyclone (Logan, UT). A BacLight Live/Dead viability kit was purchased from Molecular Probes (Eugine, OR). Toray carbon paper (TGP-H-090) was purchased from Shanghai Chuxi Industrial Co. Ltd. The 96-well cell culture plates were obtained from Nalge Nunc International (Rochester, NY). The OD₆₀₀ (optical density at 600 nm) and OD₅₉₀ of S. oneidensis MR-1 were read on a microplate reader (Bio-Tek Synergy HT, USA). Zeta potentials were determined using a Nano ZS90 (Malvern, UK). CLSM characterization was made with a confocal laser scanning biological microscope (FV1000-IX81, Olympus, Japan). SEM images were taken from Hitachi S-4800 SEM. EIS and CV were carried out using the Autolab PGATAT302N (Metrohm, Switzerland) and Shanghai Chenhua electrochemical workstation, respectively. The current-time curves were measured using Keithley 2450. The electrodeposition was performed using the Autolab PGATAT302N (Metrohm, Switzerland). The sweat, urine, and plasma were purchased from Beijing Huizhi Taikang Co. Ltd. All other chemicals were of analytical reagent grade and used as purchased without further purification.