Hemin hm

Manufactured by Merck Group

Hemin (Hm) is a chemical compound that serves as a cofactor for various enzymes. It is derived from heme, a component of hemoglobin. Hemin plays a crucial role in various biological processes, including oxygen transport, energy production, and cellular respiration.

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Lab products found in correlation

Feso4, by Merck Group (2 mentions) Hemoglobin hb, by Merck Group (2 mentions) 2 2 dipyridyl dp, by Merck Group (2 mentions)

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Hemin (689 citations)

2 protocols using hemin hm

Bacterial Cultivation and Iron Regulation

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The bacterial strains and plasmids used in this work are indicated in Table 1. E. coli strains were cultured in Luria-Bertani (LB) medium at 37°C. C. violaceum strains were cultured in LB medium or M9 minimal medium supplemented with 0.1% casein hydrolysate (M9CH) at 37°C (Batista et al., 2019 (link)). The cultures were supplemented with kanamycin (50 μg/mL), tetracycline (10 µg/mL), or ampicillin (100 μg/mL), when necessary. Iron deficiency was obtained by the addition of 2,2’-dipyridyl (DP) (Sigma) to the medium, while iron sufficiency was achieved by supplementation with FeSO₄ (Sigma), hemin (Hm) (Sigma), or hemoglobin (Hb) (Sigma).

de Lima V.M., Batista B.B, & da Silva Neto J.F. (2022). The Regulatory Protein ChuP Connects Heme and Siderophore-Mediated Iron Acquisition Systems Required for Chromobacterium violaceum Virulence. Frontiers in Cellular and Infection Microbiology, 12, 873536.

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Bacterial Strain Growth Conditions

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Bacterial strains, plasmids, and growth conditions. The bacterial strains and plasmids used in this work are indicated in Table 1. E. coli strains were cultured in Luria-Bertani (LB) medium at 37 C. C. violaceum strains were cultured in LB medium or M9 minimal medium supplemented with 0.1% casein hydrolysate (M9CH) at 37 C (Batista et al., 2019) (link). The cultures were supplemented with kanamycin (50 μg/mL), tetracycline (10 µg/mL), or ampicillin (100 μg/mL), when necessary. Iron deficiency was obtained by the addition of 2,2'-dipyridyl (DP) (Sigma) to the medium, while iron sufficiency was achieved by supplementation with FeSO4 (Sigma), hemin (Hm) (Sigma), or hemoglobin (Hb) (Sigma).

Lima V.M.d., Batista B.B., & Neto J.F.d.S. (2022). The regulatory protein ChuP connects heme and siderophore-mediated iron acquisition systems required for Chromobacterium violaceum virulence.

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