Purified dao from porcine kidney

Manufactured by Merck Group

Sourced in United States

Purified DAO from porcine kidney is a laboratory product that contains the enzyme diamine oxidase (DAO) isolated from porcine (pig) kidney tissue. DAO is an enzyme involved in the metabolism of biogenic amines. The product provides a source of purified DAO for use in various research and analytical applications.

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2 protocols using purified dao from porcine kidney

Quantification of Histamine and DAO

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Histamine dihydrochloride and purified DAO from porcine kidney were purchased from Sigma-Aldrich (St. Louis, MO, USA). UHPLC-grade methanol and acetonitrile, hydrochloric acid 0.1M, perchloric acid 70%, sodium di-hydrogen phosphate anhydrous and di-sodium hydrogen phosphate anhydrous were obtained from PanReac Química (Castellar del Vallès, Spain). Acetic acid, boric acid, 1-octanesulfonic acid sodium salt, phthaldialdehyde (OPA) and brij® L23 solution were acquired from Sigma-Aldrich (St. Louis, MO, USA); sodium acetate anhydrous, potassium hydroxide and 2-mercaptoethanol from Merck (Darmstadt, Germany).
A LaboStar System from Evoqua Water Technologies (Warrendale, PA, USA) was used to produce ultrapure water (18.2 MΩcm).

Comas-Basté O., Latorre-Moratalla M.L., Rabell-González J., Veciana-Nogués M.T, & Vidal-Carou M.C. (2020). Lyophilised legume sprouts as a functional ingredient for diamine oxidase enzyme supplementation in histamine intolerance. Lebensmittel-Wissenschaft + [i.e. und] Technologie. Food science + technology. Science + technologie alimentaire, 125.

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Quantification of Biogenic Amines in Foods

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Histamine dihydrochloride, purified DAO from porcine kidney and catalase from bovine liver were purchased from Sigma-Aldrich (St. Louis, MO, USA). UHPLC-grade methanol and acetonitrile, hydrochloric acid 0.1M, perchloric acid 70%, sodium di-hydrogen phosphate anhydrous and di-sodium hydrogen phosphate anhydrous were obtained from PanReac Química (Castellar del Vallès, Spain). Acetic acid, boric acid, 1-octanesulfonic acid sodium salt, ammonium formate, phthaldialdehyde (OPA) and brij® L23 solution were acquired from Sigma-Aldrich (St. Louis, MO, USA); and formic acid, sodium acetate anhydrous, potassium hydroxide and 2-mercaptoethanol from Merck (Darmstadt, Germany). A LaboStar System from Evoqua Water Technologies (Warrendale, PA, USA) was used to produce ultrapure water (18.2 MΩcm).

Comas-Basté O., Latorre-Moratalla M.L., Sánchez-Pérez S., Veciana-Nogués M.T, & Vidal-Carou M.C. (2019). In vitro determination of diamine oxidase activity in food matrices by an enzymatic assay coupled to UHPLC-FL. Analytical and bioanalytical chemistry, 411(28).

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