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Lipase from rhizomucor miehei

Manufactured by Merck Group

Lipase from Rhizomucor miehei is a microbial enzyme that hydrolyzes ester bonds in lipids. It is a widely used biocatalyst in various industrial applications.

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2 protocols using lipase from rhizomucor miehei

1

Radiolabeled Acetate Metabolism

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Unless specified, all chemicals were purchased from Fisher Scientific (www. fishersci.com), and solvents were at least HPLC grade. [ 14 C]acetate sodium salt 50 mCi/mmol was from American Radiolabeled Chemicals, Inc. Glass thinlayer chromatography (TLC) plates (hard-layer 250 mm, 20 3 20 cm) were from Analtech. The liquid scintillation fluid was EcoScint Original (National Diagnostics). Lipase from Rhizomucor miehei and phospholipase A2 from Apis mellifera were purchased from Sigma-Aldrich.
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2

Enzymatic Biodiesel Production from Diverse Oils

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Methyl esters of the major fatty acids and lipase from Rhizomucor miehei (solution) were purchased from Sigma-Aldrich. Jatropha oil was purchased from Yanyuan County, China. Olive oil, soybean oil, canola oil, castor oil and Rhodamine B were purchased from Aladdin (shanghai, China). All other reagents were of analytical grade and used without further puri cation. Double distilled water was employed throughout the experiments.
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