Glutathione peroxidase gsh px assay kit

For cell cultures, DMEM, FBS, penicillin, streptomycin, and trypsin-EDTA were purchased from GE Life (HyClone, USA). DMSO, MTT, MTS, and Hoechst 33258 were purchased from Sigma (Santa Clara, USA). An annexin V-FITC/PI apoptosis detection Kit, reactive oxygen species (ROS) assay kit, malondialdehyde (MDA) assay kit, glutathione peroxidase (GSH-Px) assay kit and superoxide dismutase (SOD) assay kit were purchased from KeyGen Biotech (Beijing, CN).The primary antibodies against β-actin, Bax, Bcl-2, Caspase-9, Caspase-3, Cyt C, and Vadc 1 were purchased from Cell Signaling (Massachusetts, USA). Horseradish peroxidase-conjugated goat anti-rabbit antibody was also purchased from Cell Signaling and was used as the secondary antibody. All the other fine chemicals and solvents used for this study were of analytical grade.

Tannic acid was purchased from Shanghai Macklin Biochemical Technology Co., Ltd., (Shanghai, China). MTT was obtained from Solarbio (Beijing, China). The bicinchoninic acid (BCA) protein assay kit, LDH cytotoxicity assay kit, superoxide dismutase (SOD) assay kit, catalase (CAT) assay kit, glutathione peroxidase (GSH-PX) assay kit, lipid peroxidation (MDA) assay kit, and ATP assay kit were purchased from Nanjing KeyGen Biotech. Co. Ltd (Nanjing, China). The Annexin V-FITC apoptosis detection kit and mitochondrial membrane potential assay kit were obtained from Nanjing KeyGen Biotech. Co. Ltd. The ROS content assay kit was purchased from Beyotime Biotech. Co. Ltd. (Nantong, China). Rabbit monoclonal antibody of Bcl-2, Bax, PARP-1, caspase-3, caspase-9, cytochrome c, P53, ATM, P-ATM. CDK, P-CDK, cyclin A2, and β-actin, and goat anti-rabbit antibody of IgG-HRP were obtained from Cell Signaling Technology (Boston, MA, USA). Z-LEHD-FMK was purchased from Abcam, Inc. (Cambridge, UK). The 8-OHdG assay kit was purchased from Elabscience Biotechnology Co., Ltd. (Wuhan, China).