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Acrylamide

Manufactured by Sisco Research Laboratories
Sourced in India

Acrylamide is a chemical compound used in laboratory equipment. It is a white, crystalline solid with a high water solubility. Acrylamide is commonly used as a monomer in the synthesis of polyacrylamide, which is a key component in various laboratory techniques and applications.

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4 protocols using acrylamide

1

Biochemical Assay Reagents and Protocols

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Thiobarbituric acid (TBA), bovine serum albumin (BSA), Sephadex G-25, and 5,5′ dithiobis 2-nitrobenzoic acid (DTNB) were purchased from Sigma Chemical Co., USA. NN′-bis-acrylamide and NNN′N′-tetramethylethylenediamine (TEMED) were purchased from Merk-Schudart, and reduced glutathione, ascorbic acid, horse radish peroxidase, hydrogen peroxide, 2,4-dinitrophenylhydrazine (DNPH), guanidine hydrochloride, acrylamide, sodium dodecyl sulfate (SDS), ferric chloride, potassium ferricyanide, and ammonium persulfate were obtained from SISCO Research Laboratory, India. Digitonin was procured from Hi-media Ltd., India. All other chemicals used were of analytical grade.
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2

Sumatriptan Succinate Transdermal Formulation

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Sumatriptan succinate was purchased from Aurobindo pharmaceutical, India. HPMC was obtained from Colorqon Asia Pvt. ltd., India. PEG was acquired from Molychem laboratory, India. Xanthan gum was procured from Purix global, India. Potassium persulphate was purchased from Loba Chemie Pvt. ltd, India. Acrylamide was acquired from Sisco research laboratories Pvt. ltd., India, and acetone were obtained from Merck Life science Pvt. ltd., India. All other materials used for analysis were of analytical grade.
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3

Western Blot Reagents and Suppliers

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Bovine serum albumin (BSA), glycine, protease inhibitor cocktail, RA and Tween 20 were from Sigma-Aldrich (St. Louis, MO, USA). CP was procured from Dr Reddy’s Laboratories (Hyderabad, India). Primary and secondary antibodies were purchased from Cell Signaling Technology, Inc. (Danvers, MA, USA), Santa Cruz Biotechnology, Inc. (Dallas, TX, USA) and Elabscience (Houston, TX, USA). Eosin, hematoxylin, Ponceau S, β-mercaptoethanol and Triton X-100 were purchased from Hi-Media Laboratories Pvt. Ltd. (Mumbai, India). Acrylamide, bisAcrylamide, ethylene-diaminetetraacetic acid and tris-hydroxymethylaminomethane were purchased from Sisco Research Laboratories Pvt. Ltd. (Mumbai, India). Horseradish peroxidase was from E. Merck Ltd. (Mumbai, India). Other routine chemicals and reagents were purchased from either Sigma-Aldrich or accredited local vendors.
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Isolation and Characterization of Penicillium chrysogenum SNP5

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Penicilliumchrysogenum SNP5 (MTCC13144) strain was locally isolated from grease-contaminated soil of the diesel loco shed and identified by Microbial Type Culture Collection and gene bank Chandigarh, India23 (link). Triton X-100, Phenylmethylsulfonyl fluoride (PMSF), Lauryl dimethylamine oxide (LDAO), Nicotinamide adenine dinucleotide (NAD) + hydrogen (NADH), 1, 6- diphenyl- 1, 3, 5- hexatriene (DPH), Acrylamide, Bis Acrylamide, Coomassie brilliant blue R-250, Glycine, Bromophenol blue, Methanol, para-nitrophenol, 1-Bromooctane, Dimethyl Sulphoxide (DMSO), Petroleum ether, diethyl ether, other medium components, and Czapek-dox medium were procured from Sisco Research Laboratories Pvt. Ltd. (Mumbai, India). Hexadecane and THB (Tetrahydrobiopterin), Acetic acid, and Digitonin were procured from TCI Chemicals Pvt. Ltd. (India). Phosphatidylcholine and cholesterol were purchased from Sigma-Aldrich Chemicals Pvt. Ltd. PageRuler™ Plus Prestained Protein Ladder was procured from Thermo Scientific.
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