Antibiotic antimycotic

Manufactured by Cytiva

Sourced in United States

Antibiotic-antimycotic is a solution containing a combination of antibiotics and antifungal agents. It is designed for use in cell culture applications to prevent bacterial and fungal contamination.

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Lab products found in correlation

Rpmi 1640 medium, by Thermo Fisher Scientific (3 mentions) Umuc3, by American Type Culture Collection (3 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (3 mentions) Sv huc 1, by American Type Culture Collection (3 mentions) Rpmi 1640 medium, by Cytiva (2 mentions) Antibiotic antimycotic, by Thermo Fisher Scientific (2 mentions) Fetal bovine serum (fbs), by Cytiva (2 mentions) Mem vitamin solution, by Cytiva (2 mentions) Image it fixation permeabilization kit, by Thermo Fisher Scientific (1 mentions) Anti e cadherin, by BD (1 mentions) A549 cell line, by American Type Culture Collection (1 mentions) Dulbecco modified eagle medium (dmem), by Cytiva (1 mentions) Dulbecco s modified eagle medium, by Cytiva (1 mentions)

Close spelling variants of this product

Antibiotic-antimycotic solution (7 citations)

9 protocols using antibiotic antimycotic

Microbial Growth Media Preparation

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BBL Mueller Hinton II Broth
CationAdjusted (500 g, Lot #: 9324795), Difco Luria–Bertani
(LB) Broth Miller Luria–Bertani (500 g, Lot #: 0365816), Bacto
Tryptic Soy Broth (500 g, Lot #: 5254874), and Difco Tryptic Soy Agar
(500 g, Lot #: 9050734) were purchased from BD Bioscience. RPMI 1640
medium (500 mL, Catalog #: 11875093), Hyclone Charaterized FBS, US
Origin (500 mL, Catalog #: SH30073.03), and Antibiotic Antimycotic
(100×, 100 mL, Catalog #: 15240062) were purchased from Cytiva.
Complete RPMI medium was made by adding 10% of heat-inactivated FBS
and 1% of Antibiotic Antimycotic.

Choi Y., Choe H.W., Kook M., Choo S., Park T.W., Bae S., Kim H., Yang J., Jeong W.S., Yu J., Lee K.R., Kim Y.S, & Yu J. (2023). Proline-Hinged α-Helical Peptides Sensitize Gram-Positive Antibiotics, Expanding Their Physicochemical Properties to Be Used as Gram-Negative Antibiotics. Journal of Medicinal Chemistry, 67(3), 1825-1842.

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Establishing Colorectal Normal Fibroblasts

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Colonic NFs were established based on previously published protocols.
²⁰ (link),
²⁷ (link),
²⁸ (link) To obtain the tissue, during en bloc resection of specimens after colorectal ESD, normal mucosa was excised and washed in PBS with 1% antibiotic‐antimycotic (Thermo Fisher Scientific, Waltham, MA, USA). Dulbecco's Modified Eagle Medium (DMEM)/Ham's F‐12 with L‐Glutamine and Phenol Red (Thermo Fisher Scientific) plus 1% antibiotic‐antimycotic and 20% fetal bovine serum (FBS; Cytiva, Tokyo, Japan) was used as the medium. As the demarcation between the tumor and non‐tumor regions of colorectal tumors is visibly discernible through the naked eye or endoscopic observation, we collected normal mucosa specifically from areas identified as tumor‐free by visual assessment. NFs were subjected to fluorescent immunostaining using Image‐iT Fixation/Permeabilization Kit (Thermo Fisher Scientific) and observed using a confocal laser microscope (C2si; Nikon Corporation, Tokyo, Japan). Anti‐α‐smooth muscle (αSMA; 14‐9760‐80; dilution 1:1000; Thermo Fisher Scientific) and anti‐E‐cadherin (610181; dilution 1:1000; Becton, Dickinson and Company, Franklin Lakes, NJ, USA) were used as primary antibodies, and Donkey anti‐Mouse IgG (H + L) Highly Cross‐Adsorbed Secondary Antibody, Alexa Fluor™ 488 (A‐21202, dilution 1:2000; Thermo Fisher Scientific) was used as a secondary antibody.

Inomata Y., Kuroha M., Shimoyama Y., Naito T., Moroi R., Shiga H., Kakuta Y., Karasawa H., Onuma S., Kinouchi Y, & Masamune A. (2024). Dickkopf 1 is expressed in normal fibroblasts during early stages of colorectal tumorigenesis. Cancer Medicine, 13(2), e6992.

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Bladder Cancer Cell Lines Cultivation

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The human BCa cell lines T24, UM-UC-3, 5637, and 253 J and the normal urothelium cell line SV-HUC-1, which is an SV-40 immortalized human uroepithelial cell line, were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA). The cell lines mentioned above (except for 5637) were maintained in DMEM culture medium, and 5637 cells were cultured in RPMI-1640 medium supplemented with 10% FBS (Gibco, Grand Island, NY, USA) and 1% antibiotic-antimycotic (HyClone Laboratories, Logan, UT) at 37 °C aired with 5% CO₂ and 95% humidity in a cell incubator. The T24-L subcellular line containing luciferase was generated as described in previous research [10 (link)] and cultured in DMEM under the same conditions described above. All cell lines used in the research were at early passages.

Zhang M., Wang L., Yue Y., Zhang L., Liu T., Jing M., Liang X., Ma M., Xu S., Wang K., Wang X, & Fan J. (2021). ITPR3 facilitates tumor growth, metastasis and stemness by inducing the NF-ĸB/CD44 pathway in urinary bladder carcinoma. Journal of Experimental & Clinical Cancer Research : CR, 40, 65.

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Bladder Cancer Cell Lines Cultivation

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The human BCa cell lines T24, UM-UC-3, 5637, and 253J and the normal urothelium cell line SV-HUC-1, which is an SV-40 immortalized human uroepithelial cell line, were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA). The cell lines mentioned above (except for 5637) were maintained in DMEM culture medium, and 5637 cells were cultured in RPMI-1640 medium supplemented with 10% FBS (Gibco, Grand Island, NY, USA) and 1% antibiotic-antimycotic (HyClone Laboratories, Logan, UT) at 37°C aired with 5% CO 2 and 95% humidity in a cell incubator. The T24-L subcellular line containing luciferase was generated as described in previous research 10 and cultured in DMEM under the same conditions described above. All cell lines used in the research were at early passages.

Zhang M., Wang L., Yue Y., Zhang L., Liu T., Jing M., Liang X., Ma M., Xu S., Wang K., Wang X., & Fan J. (2020). ITPR3 Facilitates Tumor Growth, Metastasis and Stemness by Inducing the NF-ĸB/CD44 Pathway in Urinary Bladder Carcinoma.

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Bladder Cancer Cell Lines Cultivation

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The human BCa cell lines T24, UM-UC-3, 5637, and 253J and the normal urothelium cell line SV-HUC-1, which is an SV-40 immortalized human uroepithelial cell line, were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA). The cell lines mentioned above (except for 5637) were maintained in DMEM culture medium, and 5637 cells were cultured in RPMI-1640 medium supplemented with 10% FBS (Gibco, Grand Island, NY, USA) and 1% antibiotic-antimycotic (HyClone Laboratories, Logan, UT) at 37°C aired with 5% CO 2 and 95% humidity in a cell incubator. The T24-L subcellular line containing luciferase was generated as described in previous research 10 and cultured in DMEM under the same conditions described above. All cell lines used in the research were at early passages.

Zhang M., Wang L., Yue Y., Zhang L., Liu T., Jing M., Zhang Y., Ma M., Xu S., Wang K., Wang X., & Fan J. (2021). ITPR3 facilitates tumor growth, metastasis and stemness by inducing the NF-ĸB/CD44 pathway in urinary bladder carcinoma.

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MDA-MB-231 Cell Culture Protocol

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MDA-MB-231 cells were donated by Prof. Luo Bing (Department of Microbiology, Qingdao University, Qingdao, Shandong, China). Cells were cultured in RPMI 1640 medium supplemented with 10% fetal bovine serum (FBS) and 1% antibiotic-antimycotic (Hyclone Laboratories, Inc., Logan UT) at 37℃ in a 5% CO 2 atmosphere.

Yang Z., Ji L., Jiang G., Liu R., Liu Z., Yang Y., Ma Q, & Zhao H. (2018). FL118, a novel camptothecin analogue, suppressed migration and invasion of human breast cancer cells by inhibiting epithelial-mesenchymal transition via the Wnt/β-catenin signaling pathway. Bioscience trends, 12(1).

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Culturing Fibroblasts and PBMCs

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Fibroblasts were grown in Minimal Essential Medium (MEM) supplemented with 2X concentration of essential and non-essential amino acids for MEM, 1X MEM vitamin solution, 1X antibiotic/antimycotic, and 10% fetal bovine serum from Hyclone Laboratories, Inc. All other cell culture solutions were purchased from Invitrogen. Upon confluence, cells were removed from culture flasks by incubating for 1 minute in 0.7 mM EDTA in Dulbecco’s phosphate buffered saline (pH 7.4), and then for 2–5 minutes in 0.05% trypsin. Cells were then washed with phosphate buffered saline/EDTA and used for experiments, or passaged in a ratio appropriate to the culture’s growth rate. Cells were cultured from passages 3 to 7 (depending on the passage number of the original stock) to passage 14. No passage-dependent metabolic changes were observed in any of the cell lines. All experiments were performed in serum free media.
Alternatively, 25 ml of peripheral blood was collected into heparinized tubes by venous puncture and PBMCs were purified by histopaque 1077. PBMCs were archived in 90% fetal calf serum/10% DMSO at -80 or liquid nitrogen and analyzed within 3 months, then thawed and cultured as described [25 (link)].

Jones IV A.R., Coleman E.L., Husni N.R., Deeney J.T., Raval F., Steenkamp D., Dooms H., Nikolajczyk B.S, & Corkey B.E. (2017). Type 1 diabetes alters lipid handling and metabolism in human fibroblasts and peripheral blood mononuclear cells. PLoS ONE, 12(12), e0188474.

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Culturing NSCLC, RAW 264.7, and PDCs

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Non-small-cell lung cancer (NSCLC) A549 cell lines were purchased from the American Type Culture Collection (Manassas, Virginia, USA). A549 cells were cultured in RPMI1640 medium (HyClone Laboratories, Logan, Utah, USA) supplemented with 10% fetal bovine serum, and 1% antibiotic-antimycotic (Gibco, Waltham, Massachusetts, USA). Cell lines were incubated in a 5% CO₂-humidified atmosphere at 37°C. RAW 264.7 cells were grown in Dulbecco’s Modified Eagle Medium (DMEM) (HyClone Laboratories) with 10% fetal bovine serum and 1% antibiotic-antimycotic. Patient tissues were dissociated at 37°C with collagenase, and then patient-derived cells (PDCs) were grown in DMEM supplemented with 10% fetal bovine serum and 1% antibiotic-antimycotic.

Choi J.Y., Lee Y.S., Shim D.M, & Seo S.W. (2020). Effect of GNAQ alteration on RANKL-induced osteoclastogenesis in human non-small-cell lung cancer. Bone & Joint Research, 9(1), 29-35.

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Cell Culture in Supplemented MEM

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Cells were grown in Minimal Essential Medium (MEM) with Earle’s salts, 2X concentration of essential and non-essential amino acids for MEM, 1X MEM vitamin solution, 1X antibiotic/antimycotic, and 20% fetal bovine serum (FBS) from Hyclone Laboratories, Inc. (Logan, UT). All other cell culture solutions were purchased from Gibco Life Technologies (Gaithersburg, MD). Upon confluence, cells were removed from culture flasks by incubating for 1 minute in 0.7 mM EDTA in Dulbecco’s phosphate buffered saline (PBS; pH 7.4), and then for 2–5 minutes in 0.25% trypsin. Cells were then washed with PBS/EDTA and used for experiments, or passaged in a ratio appropriate to the culture’s growth rate.

Husni N.R., Jones IV A.R., Simmons A.L, & Corkey B.E. (2014). Fibroblasts From Type 1 Diabetics Exhibit Enhanced Ca2+ Mobilization after TNF or Fat Exposure. PLoS ONE, 9(1), e87068.

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