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Rvfv nucleoprotein

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The RVFV Nucleoprotein is a laboratory reagent used for research purposes. It is a recombinant protein derived from the Rift Valley fever virus (RVFV) nucleoprotein. The core function of this product is to provide a source of the RVFV nucleoprotein for use in various research and analytical applications.

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Lab products found in correlation

Hrp conjugated actin, by Abcam (2 mentions) Gapdh, by Cell Signaling Technology (2 mentions) Lamin a c, by Cell Signaling Technology (2 mentions) Pakt1 ser 473, by Cell Signaling Technology (1 mentions) Ifit2, by Abcam (1 mentions) Viperin, by Cell Signaling Technology (1 mentions) Total akt1, by Cell Signaling Technology (1 mentions) Cleaved caspase 3, by Cell Signaling Technology (1 mentions) Flag tag (flag), by Merck Group (1 mentions) Total stat3, by Cell Signaling Technology (1 mentions) P stat3 y705, by Cell Signaling Technology (1 mentions)

2 protocols using rvfv nucleoprotein

1

Western Blot Analysis of Protein Lysates

Cited 1 time
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Protein lysates were collected and analyzed by western blot as previously described24 (link). In brief, primary antibodies against GAPDH (Cell Signaling, 5174), Lamin A/C (Cell Signaling, 4777), p-Akt1 Ser 473 (Cell Signaling, 4060), total Akt1 (Cell Signaling, 4691), IFIT2 (Abcam - ab113112), Viperin (Cell Signaling -13996 S), Actinin 2 (Thermo Fisher Scientific - PA5-27863), Alpha-Pix (Cell Signaling -4753 S), RVFV Nucleoprotein (BEI Resources, NR-43188), or HRP-conjugated actin (Abcam, ab49900) were diluted in 3% milk solution per the manufacturer’s recommended dilutions followed by the addition of the appropriate secondary antibody. Cropped, representative images are shown in main figures, but uncropped originals can be found in the Supplemental Figure 8.
Pinkham C., Dahal B., de la Fuente C.L., Bracci N., Beitzel B., Lindquist M., Garrison A., Schmaljohn C., Palacios G., Narayanan A., Campbell C.E, & Kehn-Hall K. (2017). Alterations in the host transcriptome in vitro following Rift Valley fever virus infection. Scientific Reports, 7, 14385.
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2

Western Blot Analysis of Protein Lysates

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Protein lysates were collected and analyzed by western blot, or cellular fractionation was performed and analyzed by western blot, as previously described (Austin et al., 2012 (link)). In brief, primary antibodies against p-STAT3 Y705 (Cell Signaling, 9145S), total STAT3 (Cell Signaling, 12640S), cleaved Caspase-3 (Cell Signaling, 9661S), Flag (Sigma, F1804), RVFV Nucleoprotein (BEI Resources, NR-43188), Lamin A/C (Cell Signaling, 4777S), GAPDH (Cell Signaling, 5174S), or HRP-conjugated actin (Abcam, ab49900) were diluted in 3% milk solution according to the manufacturer’s recommended dilutions followed by the addition of the appropriate secondary antibody.
Pinkham C., An S., Lundberg L., Bansal N., Benedict A., Narayanan A, & Kehn-Hall K. (2016). The Role of Signal Transducer and Activator of Transcription 3 in Rift Valley Fever Virus Infection. Virology, 496, 175-185.
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