Caco 2 human epithelial colorectal adenocarcinoma cells

All cells were grown in 75 cm² flasks at 37°C with 5% CO₂ in humidified atmospheric pressure. Cells were spilt into new flasks once or twice per week. All culture media were supplemented with 1 IU mL⁻¹ penicillin, 1 µg mL⁻¹ streptomycin and 10% foetal bovine serum. HepG2 human liver hepatocellular carcinoma cells (passage 104–124) were obtained from Health Protection Agency Culture Collections (Porton Down, U.K.) and grown in William's E Medium Glutamax. CaCo-2 human epithelial colorectal adenocarcinoma cells (passage 31–97), originating from the American Type Culture Collection (Manassas, VA, U.S.A.) were cultured in Dublecco's Modified Eagle Medium (DMEM), 1% non essential amino acids (NEAA), and 2 mM L-glutamine. SH-SY5Y human neuroblastoma cells (passage 28) obtained from European Collection of Cell Cultures (ECACC; Porton Down, UK) were cultured in MEM with Earl's salts, 1% NEAA, 1% L-glutamine. Rat C6 glioma cells (passage range 18–22) obtained from ECACC were cultured in Ham's F-10 medium with 2 mM glutamine. PC-3 human prostate cancer cells (passage range 18–20) were obtained from DSMZ (Braunschweig. Germany) and cultured in Ham's F-10 medium, 2 mM L-glutamine, 10% foetal bovine serum and penicillin + streptomycin. Media and all supplements were obtained from Invitrogen Life technologies (Stockholm, Sweden).