Venusil innova durashell nh2 column

A pH meter model HI2221 (HANNA Instruments, Padua, Italy) was used to measure the pH values of seven goji berry pulp samples, and a digital display saccharimeter model 2818 (Spectrum, Stamford, CT, USA) was used to measure the soluble solids at room temperature. The high-performance liquid chromatography (HPLC) method previously published in our study was used to examine the organic acids, such as lactic acid, tartaric acid, oxalic acid, citric acid, and malic acid, and reducing sugars, such as glucose and fructose [52 (link)]. A Venusil XSB C18 column (4.6 mm × 250 mm, 5 μm; Bonna-Agela Technologies Co. Ltd., Tianjin, China) and a PDA detector set at 210 nm were included in the Shimadzu LC-20AT system (Shimadzu, Kyoto, Japan), which measured the concentration of organic acids. A Venusil Innova Durashell NH₂ column (4.6 mm × 250 mm, 5 μm; Bonna-Agela Technologies Co. Ltd., Tianjin, China), a Shimadzu LC-20AT HPLC, and an RID-20A detector (Shimadzu, Japan) were used to examine reducing sugars. Each analysis was performed in triplicate for each sample.

A Shimadzu LC-20AT high performance liquid chromatograph (Shimadzu, Japan), equipped with a RID-20A detector (Shimadzu, Japan), was used to analyze the sugar consumption in the juice after inoculating lactic acid bacteria. A Venusil Innova Durashell NH2 column (4.6 × 250 mm, 5 µm, Bonna-Agela Technologies Co. Ltd., Tianjin, China) was used to separate glucose and fructose. The analysis program followed the instruction of the chromatographic column manufacture. The mobile phase was comprised of (A) acetonitrile and (B) water. The column temperature was maintained at +25 °C and the elution was set at an isocratic flow rate of 0.8 mL/min (A) and 0.2 mL/min (B) for 13 min with an injection volume of 20 µL of the filtered juices. The external standard glucose and fructose were used to identify and quantify the compounds in the juices.