Human colon carcinoma HT-29 cells were purchased from the Cell Bank of the Chinese Academy of Sciences (Shanghai, China). Cells were grown as adherent monolayers in DMEM culture media containing 10% (v/v) FBS, 100 U/ml penicillin and 100 μg/ml streptomycin at 37°C in a humidified incubator with an atmosphere of 5% CO2.
Ht 29 cells
Manufactured by National Collection of Authenticated Cell Cultures
Sourced in China
HT-29 cells are a well-established human colon adenocarcinoma cell line. They are commonly used in cell biology and cancer research applications.
Automatically generated - may contain errors
5 protocols using ht 29 cells
1
Culturing Human Colon Carcinoma Cells
2
Exopolysaccharide-Producing Lactobacillus Strain
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The EPS-producing strain L. plantarum WLPL04 was previously isolated from healthy female breast milk (Jiang et al., 2016) , and cultured in de Man, Rogosa, and Sharpe broth (Beijing Solarbio Science and Technology Co. Ltd., Beijing, China) at 37°C under anaerobic condition. Indicator strains of pathogens (Pseudomonas aeruginosa CMCC10104, Escherichia coli O157:H7, Salmonella Typhimurium ATCC13311, and Staphylococcus aureus CMCC26003) were cultured in Luria-Bertani medium at 37°C overnight in a shaker incubator.
Human colon cancer HT-29 cells, purchased from Cell Bank of the Chinese Academy of Sciences (Beijing, China), were cultured in Dulbecco's modified Eagle's medium supplemented with 10% (vol/vol) heatinactivated fetal bovine serum (ExCell Bio Co., Ltd., Shanghai, China) and a standard mixture of antibiotics (100 U/mL of penicillin, 100 µg/mL of streptomycin; Beijing Solarbio Science and Technology Co. Ltd.) in an atmosphere of 5% (vol/vol) CO 2 at 37°C, in 75-cm 2 cell culture flasks, and the medium was replaced every 2 d.
Human colon cancer HT-29 cells, purchased from Cell Bank of the Chinese Academy of Sciences (Beijing, China), were cultured in Dulbecco's modified Eagle's medium supplemented with 10% (vol/vol) heatinactivated fetal bovine serum (ExCell Bio Co., Ltd., Shanghai, China) and a standard mixture of antibiotics (100 U/mL of penicillin, 100 µg/mL of streptomycin; Beijing Solarbio Science and Technology Co. Ltd.) in an atmosphere of 5% (vol/vol) CO 2 at 37°C, in 75-cm 2 cell culture flasks, and the medium was replaced every 2 d.
3
HT-29 Cells Maintenance Protocol
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Human colon carcinoma cells (HT-29 cells) were purchased from the cell bank of the Chinese Academy of Sciences (Shanghai, China), which were respectively maintained in Dulbecco’s Modified Eagle Medium/F12 medium (DMEM/F12) supplemented with 10% (v/v) fetal bovine serum (FBS, Biological Industries, Kibbutz Beit-Haemek, Israel) under a humidified atmosphere of 5% CO2 humidified air at 37°C.
4
Culturing Human Colon Cancer Cells
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Human colon cancer HT-29 cells were purchased from the Type Culture Collection of the Chinese Academy of Sciences. HT-29 cells were maintained in sterile Dulbecco's Modified Eagle Medium (DMEM) supplemented with 10% FBS, 100 U/mL penicillin, 100 μg/mL streptomycin, and 2 mM Gln-glutamine at 37°C. Cells were plated on a 24-well plate, 96-well plate, or 100-mm dish at a density of 2 × 105 cells/cm2, incubated in a CO2 incubator at 37°C for 24 h, and then treated with compounds dissolved in culture medium. Experiments were done using cells that had previously been passaged five to fifteen times.
5
Culturing Human Colon Cancer HT-29 Cells
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Human colon cancer HT-29 cells were purchased from the Type Culture Collection of the Chinese Academy of Sciences, Shanghai, China. HT-29 cells were cultured in sterile Dulbecco's modified Eagle's medium (Hyclone, UT, USA) supplemented with 10% FBS and 1% penicillin-streptomycin under a humidified incubator with 5% CO2 at 37°C.
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