A1207

The human ovarian cancer cell line SKOV3 was purchased from the Korean Cell Line Bank. The cells were cultured and maintained in the Roswell Park Memorial Institute-1640 (RPMI-1640) medium (Gibco, Grand Island, NY, USA) supplemented with 10% fetal bovine serum (FBS) (HyClone, Logan, UT, USA), 1% penicillin/streptomycin (HyClone), and 25 mM HEPES (Gibco).
Human glioblastoma (GBM) cell lines A1207, LN18, LN229, T98G, and U87MG and the human cervical cancer cell line HeLa were purchased from the American Type Culture Collection (ATCC). These cell lines were cultured and maintained in Dulbecco’s modified Eagle’s medium (DMEM) with high glucose (4500 mg/L) (HyClone), supplemented with 10% FBS, 1% penicillin/streptomycin, 2 mM L-glutamine, and 50 µg/mL gentamicin (Tocris, Bristol, UK). All cells were incubated and maintained at 37 °C in a humidified 5% CO₂ chamber.

Three TP53 mutant glioblastoma cell lines (U373MG, LN18, and U251MG) and three TP53 wild-type glioblastoma cell lines (A1207, DBTRG-05MG, and U87MG) were either purchased from the American Type Culture Collection (ATCC) or kindly provided by Prof. Shin-Hyuk Kang at Korea University, Seoul, Korea. Cells were grown in DMEM with 10% fetal bovine serum and 1% antibiotics. Tumor specimens were obtained from adult glioblastoma patients who underwent surgery at Samsung Medical Center and informed consent was received. Samsung Medical Center Institutional Review Board approved this study (IRB file #201512092). Patient-derived glioblastoma cells were grown as previously described^{28 (link)}. Briefly, surgical samples were enzymatically dissociated into single cells and cultured in neurobasal-A media (Gibco) supplemented with N2, B27, 1X l-Glutamin (Gibco), 1% penicillin/streptomycin (Gibco), human recombinant bFGF and EGF.

The patient-derived GSCs 84NS (RRID: CVCL_C6J0) and 528NS (RRID: CVCL_C6IV) were kindly provided by Dr. Ichiro Nakano (University of Alabama, Birmingham, AL, USA). The cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM)/F12 (GE Healthcare, Chicago, IL, USA) supplemented with 0.2% B27 (Invitrogen, Carlsbad, CA, USA), 20 ng/mL epidermal growth factor (EGF) (R&D Systems, Minneapolis, MN, USA), 20 ng/mL basic fibroblast growth factor (R&D Systems), 1% penicillin/streptomycin (GE Healthcare), 2 mmol/L L-glutamine (GE Healthcare), and 50 μg/mL gentamicin (Mediatech, Manassas, VA, USA). HEK293T (RRID: CVCL_0063), LN18 (RRID: CVCL_0392), A1207 (RRID: CVCL_8481), and A172 (RRID: CVCL_0131) cell lines were purchased from the American Type Culture Collection (Manassas, VA, USA). These cell lines were cultured in DMEM (GE Healthcare) supplemented with 10% fetal bovine serum (GE Healthcare), 1% penicillin/streptomycin, 2 mmol/L L-glutamine, and 50 μg/mL gentamicin. MG132, CQ, VER-155008, and CHX were purchased from Sigma-Aldrich (St. Louis, MO, USA). Ganetespib was purchased from Abmole Bioscience (Houston, TX, USA). Cell lines used in this study were regularly monitored to ensure the mycoplasma-free cells. In addition, all human cell lines were authenticated in 2019 using short tandem repeat profiling.