Goat anti 4hne

Manufactured by US Biological

Sourced in United States

Goat anti-4HNE is a primary antibody produced in goats and specifically designed to detect the presence of 4-hydroxynonenal (4HNE), a biomarker for oxidative stress. This antibody can be used in various immunoassay techniques to quantify 4HNE levels in biological samples.

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Lab products found in correlation

2 protocols using goat anti 4hne

Comprehensive Proteomic Profiling of Cellular Organelles

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The primary antibodies used were: mouse anti-β-actin (1:1000, sc-47778, Santa Cruz Biotechnology, Inc.), goat anti-4HNE (1:1000, H6275-02, US Biological Life Sciences), mouse anti-Total OXPHOS Human WB Antibody Cocktail (1:1000, ab110411, Abcam, Inc.), rabbit anti-GAPDH (1:1000, sc-25778, Santa Cruz Biotechnology, Inc.), mouse anti-human tau (1:1000, 2024-10-31, Dako), rabbit Anti-LaminA (1:500, sc-20680, Santa Cruz Biotechnology, Inc.), mouse anti-PSD95 (1:500, sc-32290, Santa Cruz Biotechnology, Inc.), mouse anti-synaptophysin (1:1000, sc-17750, Santa Cruz Biotechnology, Inc.), mouse anti-VDAC (1:1000, sc-390996, Santa Cruz Biotechnology, Inc), mouse anti-tau PHF-1 (phosphorylated at Ser396 and Ser394) was a gift by Dr. Peter Davies (Department of Pathology, Albert Einstein College of Medicine, NY, USA), rabbit anti-TOM70 (1:300, PA5-82508, Thermo Fisher Scientific). The fluorescent dyes used were: MitoTracker Red CM-H2Xros (Catalog number: M7513, Thermo Fisher Scientific), MitoTracker Green FM (Catalog number: M7514, Thermo Fisher Scientific), VECTASHIELD Antifade Mounting Medium with DAPI (Catalog number: H1200, Vector Laboratories, Inc).

Torres A.K., Jara C., Olesen M.A, & Tapia-Rojas C. (2021). Pathologically phosphorylated tau at S396/404 (PHF-1) is accumulated inside of hippocampal synaptic mitochondria of aged Wild-type mice. Scientific Reports, 11, 4448.

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Antibody Panel for Synaptic Proteins

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The primary antibodies used were anti-3-nitrotyrosine (A21285, Invitrogen, Waltham, USA), goat anti-4HNE (H6275-02, US Biological, Life Sciences, Salem, USA), mouse anti-Nrf2 (A-10) (sc-365949, Santa Cruz Biotechnology, Dallas, USA), mouse anti-PGC1α (4A8; sc-517380, Santa Cruz Biotechnology), mouse anti-Syt1/2 (H-9) (sc-393392, Santa Cruz Biotechnology), mouse anti-Syp (D-4; sc-17750, Santa Cruz Biotechnology), goat anti-pSyn Ia/b (Ser 553; sc-12913, Santa Cruz Biotechnology), mouse monoclonal anti-PSD95 (clon K28/43, UC Davis/NIH NeuroMab Facility, Davis, USA), mouse anti-GluN2A (E-4, sc-515148, Santa Cruz Biotechnology), mouse anti-GluN2B (A-8; sc-365597, Santa Cruz Biotechnology), mouse anti-GluA2 (clone L21/32; UC Davis/NIH NeuroMab Facility), goat anti-synaptophysin (sc-7568, Santa Cruz Biotechnology), and rabbit polyclonal anti-Arc (156 003, Synaptic Systems, Goettingen, Germany). Anti-Actin (sc-47778, Santa Cruz Biotechnology) or GAPDH (sc-25778, Santa Cruz Biotechnology) were used as the loading control.

Montecinos-Oliva C., Arrázola M.S., Jara C., Tapia-Rojas C, & Inestrosa N.C. (2020). Hormetic-Like Effects of L-Homocysteine on Synaptic Structure, Function, and Aβ Aggregation. Pharmaceuticals, 13(2), 24.

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