Carboplatin

The human NSCLC cell lines, NCI-H1568 and A549 were obtained from the American Type Culture Collection. HCC95, NCI-H3122, NCI-H1650, HCC827, NCI-H1975, normal airway epithelial cell line (SALE) and KRAS^G12D transformed SALE cells (SALER) were kindly provided by Dr. Thomas Roman. Cancer cells were cultured in RPMI-1640 medium supplemented with 10% FBS, L-glutamine, 100 U/ml penicillin and 50 μg/ml streptomycin. Cell lines were identity-verified by STR analysis and certified as mycoplasma-free within 6 months of the experiments reported. All cell lines were authenticated using the Stem-Elite ID System (Promega, Madison, WI, USA).
Tyrosine kinase inhibitors Gefitinib, Lapatinib, and Erlotinib were purchased from Vichem Chemie. Chemotherapeutic agents Paclitaxel, and putative stem cell killers Metformin and Salinomycin were purchased from Sigma. Afatinib was purchased from SelleckChem, Carboplatin was purchased from Santa Cruz.

Tyrosine kinase inhibitors Gefitinib, Lapatinib, Erlotinib, Bosutinib, Dasatinib and Sunitinib were purchased from Vichem Chemie (Hungary). The therapeutic monoclonal antibody Erbitux was purchased from the Max-Planck Pharmacy in Martinsried (Germany). Chemotherapeutic agents Paclitaxel, Gemcitabine, and putative stem cell killers Metformin and Salinomycin were purchased from Sigma (St Louis, MO). Afatinib was purchased from SelleckChem (Munich), Carboplatin was purchased from Santa Cruze (Santa Cruz, CA), and Tyrphostin AG1478 was purchased from Cell Signaling Technologies (Beverly, MA).