Elisa quantification kits

Blood samples were taken from the anterior vena cava of three pigs in each group. After the blood was tilted at room temperature for 3 h, the serum was collected by Transferpettor and stored in a centrifuge tube at −20 °C for further analysis. The serum biochemical parameters such as UN, TC, GLU, triglycerides, HDL, LDL, TP, ALB, GLO, A/G, ALT, AST, ALP, IgG, and IgM were measured with a 7600-020 Automatic Analyzer (Hitachi Ltd., Tokyo, Japan) in the Biochemical Laboratory of Zhengzhou University, Zhengzhou, China. The concentrations of GSH-Px and E₂ were respectively measured by ELISA quantification kits (Nanjing Jiancheng Bioengineering Institute, Nanjing, China).

The glucose (Glu), triglyceride (TG), total cholesterol (TC), and free fatty acid (FFA) were measured using an auto-analyzer (BMD/Hitachi 7050 Auto Analyser; Japan). Whole milk was analyzed for dry matter, protein, fat, and lactose contents by a Milkyway-cp2 rapid milk composition analyzer (Institute of Food Science and Fermentation Engineering, Zhejiang University). The cytokines (IL-8, IL-10, TNF-α) and immunoglobulin (IgG, IgA, IgM) concentrations in colostrum, milk, and serum samples were determined using specific pig-ELISA quantification kits (Nanjing Jiancheng Bioengineering Institute, Nanjing, China) according to instructions of their respective manufacturers, respectively. The serum concentrations of insulin (INS), prolactin (PRL), thyroid-stimulating hormone (TSH), leptin, and growth hormone (GH) in sows were measured by respective ELISA quantification kits (Nanjing Jiancheng Bioengineering Institute, Nanjing, China). Assessment of total antioxidant capacity (T-AOC) and total superoxide dismutase (T-SOD) in serum from sows and piglets were performed using specific assay kits (Nanjing Jiancheng Bioengineering Institute, Nanjing, China).