Roswell Park Memorial Institute (RPMI) 1640 medium (2.0 g/L, 10 mM glucose), Low fasting glucose (FG) (1.0 g/L, 5 mM) or high glucose (HG) (4.5 g/L, 25 mM) Dulbeccos's modified eagle medium (DMEM), fetal bovine serum (FBS), trypsin/ethylenediaminetetraacetic acid (EDTA), and penicillin/ streptomycin were obtained from Gibco (Grand Island, NY, USA). Metformin was obtained from Cayman (Ann Arbor, MI, USA), and used following dissolution in phosphate buffered solution (PBS). Dimethyl sulfoxide (DMSO) and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) were obtained from Sigma Chemicals (St. Louis, MO, USA). Bax, Bcl-2, TSC-2, mTOR, phospho-AMPKThr172, phospho-AMPKSer485, AMPK, phospho-Akt, Akt, and β-actin antibodies were obtained from Cell Signaling (Danvers, MA, USA). The Pro-IGF-1R and IRS-1 antibodies and goat anti-rabbit IgG-horseradish peroxidase (HRP) were obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA). The Luminate Forte Western HRP western Blotting Detection kit was obtained from Millipore (Danvers, MA, USA).
Dulbeccos s modified eagle medium dmem
Manufactured by Thermo Fisher Scientific
Sourced in Switzerland
Dulbecco's Modified Eagle Medium (DMEM) is a cell culture medium formulation that provides essential nutrients and growth factors for the maintenance and growth of various cell lines. It is a widely used basal medium in cell culture applications.
Automatically generated - may contain errors
Lab products found in correlation
Streptomycin, by Thermo Fisher Scientific (2 mentions)
Penicillin, by Thermo Fisher Scientific (2 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions)
1640 medium, by Thermo Fisher Scientific (1 mentions)
3 4 5 dimethylthiazol 2 yl 2 5 diphenyltetrazolium bromide mtt, by Merck Group (1 mentions)
Phospho akt, by Cell Signaling Technology (1 mentions)
Phospho ampk thr172, by Cell Signaling Technology (1 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (1 mentions)
Bcl2 associated x (bax), by Cell Signaling Technology (1 mentions)
Goat anti rabbit igg horseradish peroxidase hrp, by Santa Cruz Biotechnology (1 mentions)
Bcl 2, by Cell Signaling Technology (1 mentions)
β actin, by Cell Signaling Technology (1 mentions)
Luminate forte western hrp western blotting detection kit, by Merck Group (1 mentions)
Metformin, by Cayman Chemical (1 mentions)
Plasticware, by Sarstedt (1 mentions)
Niclosamide nic, by Merck Group (1 mentions)
Mefloquine mef, by Merck Group (1 mentions)
3 protocols using dulbeccos s modified eagle medium dmem
1
Metformin Effects on Cellular Signaling Pathways
2
Cell Culture Media and Reagents
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
All chemicals were purchased from Sigma-Aldrich (Buchs, Switzerland) and plastic ware was purchased from Sarstedt (Sevelen, Switzerland), unless stated otherwise. Dulbeccos’s modified Eagle medium (DMEM) and Penicillin and Streptomycin (10,000 Units/mL Penicillin, 10,000 μg/mL Streptomycin) were purchased from Gibco (Fisher Scientific AG, Reinach, Switzerland). Fetal bovine serum (FBS) and Trypsin/EDTA (0.05% Trypsin/0.02% EDTA) were purchased from Bioswisstec (Schaffhausen, Switzerland). Rat hepatoma (RH) cells (H-4-II-E) and human foreskin fibroblasts (HFFs) were from ATCC (Molsheim Cedex, France).
3
Anti-parasitic Drug Screening Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
If not stated otherwise, all chemicals were purchased from Sigma-Aldrich (Buchs, Switzerland). Dulbeccos’s modified Eagle medium (DMEM) and Penicillin and Streptomycin (10’000 Units/mL Penicillin, 10’000 μg/mL Streptomycin) were purchased from Gibco (Fisher Scientific AG, Reinach, Switzerland). Fetal bovine serum (FBS) and Trypsin/EDTA (0.05% Trypsin/ 0.02% EDTA) were purchased from Bioswisstec (Schaffhausen, Switzerland). If not stated otherwise all plastic ware was purchased from Sarstedt (Sevelen, Switzerland). ABZ, buparvaquone (BPQ), mefloquine (MEF) and niclosamide (NIC) were all purchased from Sigma-Aldrich, MMV665807 (MMV-X) was from Princeton Biomolecular Research (South Brunswick Township, New Jersey, USA) and monepantel (MPT) was obtained from LuBioScience GmbH (Zürich, Switzerland). The drugs were prepared as 10.000 parts per million (ppm) and 40 mM stocks in DMSO and stored as aliquots at -20°C.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!