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Eset setdb1

Manufactured by Merck Group
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ESET/SetDB1 is a laboratory equipment product manufactured by Merck Group. It serves as a tool for the analysis and study of epigenetic regulation processes within biological samples. The core function of ESET/SetDB1 is to facilitate the detection and quantification of specific histone methyltransferase enzymes involved in chromatin modification.

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Lab products found in correlation

H3k9me2, by Merck Group (2 mentions) H3k27me3, by Merck Group (2 mentions) H3k4me3, by Merck Group (2 mentions) H3k9ac, by Active Motif (2 mentions) Suv39h1, by Merck Group (2 mentions) H3k4me2, by Abcam (2 mentions) Chip isolation kit, by Merck Group (2 mentions) H3k4me, by Merck Group (2 mentions) G9a ehmt2 c6h3, by Abcam (2 mentions) H3k27me2, by Abcam (2 mentions) H3k9me3, by Abcam (2 mentions) Ip star, by Diagenode (1 mentions) Visionworks, by Analytik Jena (1 mentions)

Close spelling variants of this product

SETDB1 (6 citations)

2 protocols using eset setdb1

1

Chromatin Immunoprecipitation Assay Protocol

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The ChIP assays were performed using a ChIP isolation kit (Millipore, Billerica, MA). We treated 1 × 105 to 5 × 105 murine naïve T cells with 1% formaldehyde to cross-link histones to DNA. The fixed cells were sonicated to yield chromatin fragments of 200–500 base pairs (bp). The antibodies used in the ChIP assays included: H3Ac (Millipore), H4Ac (Upstate Biotechnology, Lake Placid, NY), H3K9Ac (Active Motif, Carlsbad, CA), H3K4Me3 (Millipore), H3K4Me2 (Abcam), H3K4Me (Millipore), H3K9Me2 (Millipore), H3K9Me3 (Abcam) H3K27me2 (Abcam) H3K27me3 (Millipore), p300 (Abcam), PCAF (Abcam), SUV39H1 (Millipore), CBP (Abcam), ESET/SetDB1(Millipore), Ezh2 (Cell Signaling Technology, Beverly, MA), G9a/EHMT2 (C6H3) (Abcam), HP1α (Millipore), HP1β (Active Motif), and HP1γ (Millipore). DNA was recovered by a Chelex 10% slurry and sample boiling method,10 (link) or using the IP-STAR (Diagenode, Denville, NJ) direct chip protocol followed by the IPPURE DNA purification. 1% Pre-enriched chromatin (input) served as the percentage input for sample quantification, confirmed by fold over IgG changes. The UV-exposed 4% agarose gels were imaged, and the digitized images were analyzed using the software VisionWorks (UVP). Protein signals were indexed by measuring their relative mean grey value per area.
Sarmento O.F., Svingen P.A., Xiong Y., Xavier R.J., McGovern D., Smyrk T.C., Papadakis K.A., Urrutia R.A, & Faubion W.A. (2014). A Novel Role for Kruppel-like Factor 14 (KLF14) in T-Regulatory Cell Differentiation. Cellular and Molecular Gastroenterology and Hepatology, 1(2), 188-202.e4.
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2

Chromatin Immunoprecipitation (ChIP) Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols
ChIP assays were performed using a ChIP isolation kit (Millipore). 1 × 105 to 5 × 105 murine naïve T cells were treated with 1% formaldehyde to cross-link histones to DNA. Fixed cells were sonicated to yield chromatin fragments of 200–500 bp. Antibodies used in the ChIP assays included: H3Ac (Millipore), H4Ac (upstate), H3K9Ac (Active Motif), H3K4Me3 (Millipore), H3K4Me2 (Abcam), H3K4Me (Millipore), H3K9Me2 (Millipore), H3K9Me3 (Abcam) H3K27me2 (Abcam) H3K27me3 (Millipore), p300 (Abcam), PCAF (Abcam), SUV39H1(Millipore) , CBP (Abcam), ESET/SetDB1(Millipore), Ezh2 (Cell Signalling), G9a/EHMT2 (C6H3) (Abcam), HP1α (millipore), HP1β (Active Motif), and HP1γ (Millipore ). DNA was recovered by Chelex 10% slurry and sample boiling method (Nature protocols), or utilizing the IP-STAR (diagenode) direct chip protocol followed by the IPPURE DNA purification. 1% Pre-enriched chromatin (Input) served as percent Input for sample quantification, confirmed by fold over IgG changes. UV exposed 4% agarose gels were imagined and digitized images were analyzed using the software visionworks (UVP). Protein signals were indexed by measuring their relative mean grey value per area.
Sarmento O.F., Svingen P.A., Xiong Y., Xavier R.J., McGovern D., Smyrk T.C., Papadakis K.A., Urrutia R.A, & Faubion W.A. (2014). A Novel Role for Kruppel-like Factor 14 (KLF14) in T-Regulatory Cell Differentiation. Cellular and Molecular Gastroenterology and Hepatology, 1(2), 188-202.e4.
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