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2 protocols using murideoxycholic acid

1

Characterization of Bacterial Bile Acids

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Aztreonam, cephalothin, ciprofloxacin, imipenem, metronidazole, neomycin, and vancomycin were obtained from Sigma-Aldrich (St. Louis, MO). CA, CDCA, αMCA, βMCA, DCA, LCA, and murideoxycholic acid (MDCA) were purchased from Steraloids, Inc. (Newport, RI) and Sigma-Aldrich (St Louis, MO). Other BA standards were generous gifts from Dr Alan F Hofmann (University of California, San Diego, CA). The structure and abbreviations of various BAs can be found in our previous manuscript (Zhang et al., 2012a (link)).
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2

Bile Acid Compound Sourcing Protocol

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Taurocholic acid (TCA), tauro-β-muricholic acid (TMCA), tauromurideoxycholic acid (TMDCA), taurochenodeoxycholic acid (TCDCA), taurodeoxycholic acid (TDCA), taurolithocholic acid (TLCA), cholic acid (CA), α-muricholic acid (αMCA), β-muricholic acid (βMCA), ω-muricholic acid (ωMCA), murideoxycholic acid (MDCA), ursodeoxycholic acid (UDCA), chenodeoxycholic acid (CDCA), deoxycholic acid (DCA), and lithocholic acid (LCA) were purchased from either Steraloids, Inc. (Newport, RI) or Sigma-Aldrich (St Louis, MO). Clofibric acid (CLOF) was purchased from Sigma-Aldrich Co. (St. Louis, MO). Chloroform, agarose, and ethidium bromide were purchased from AMRESCO Inc. (Solon, OH). RNA-Bee was purchased from TelTest Inc. (Friendswood, TX). All other chemicals, unless indicated, were purchased from Sigma-Aldrich Co. (St. Louis, MO).
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