Potato dextrose broth agar

D. melanogaster Canton-S flies were grown at 25° on a yeast glucose diet containing 100 g/ liter brewer’s yeast (inactive) (MP Biomedicals), 100 g/ liter glucose (Sigma), 12 g/ liter agar (Apex), and preservatives (0.04% phosphoric acid and 0.42% propionic acid (Sigma)) on a 12-h-light/12-h-dark cycle.
Bacterial strains used in the study are included in Table 1. Media used included lysogeny broth (LB)/agar (Sigma), modified MRS (mMRS) broth/agar and potato dextrose broth/ agar (Sigma). The plasmid bearing E. coli strain was cultured with 50 μg/ml kanamycin and Acetobacter gene knock-out mutants were cultured with 30 μg/ml chloramphenicol and 50 μg/ml kanamycin. All bacterial strains were cultured at 30°. A. fabarum DsW_054 was cultured in potato dextrose broth prior to matings.

21–30 dpi (days post inoculation), 1–1.5 cm of primary inflorescence stem segments were cut at the base (Additional file 1: Figure S5b), surface sterilized (5 min, 70% ethanol; 5 min 0.02% sodium hydrogen chloride solution supplemented with 0.02% Triton X-100) and thoroughly washed with germ-free water (according to [13 (link)]). These stem segments were placed on solidified Potato Dextrose Broth agar (20 g/l PDB (Sigma-Aldrich); 10 g/l phyto agar (Duchefa); Milli-Q water; autoclaved at 121 °C; supplemented after cooling to 60 °C with 500 mg/l cefotaxime (Duchefa). After incubation in darkness for 3–5 days at RT, fungal mycelium became visible.