Plan apo 100x na 1.45 oil immersion objective

Manufactured by Nikon

Sourced in Japan

The Plan Apo 100X NA 1.45 oil immersion objective is a high-performance optical lens designed for use in advanced microscopy and imaging applications. It offers a magnification of 100X and a numerical aperture of 1.45, providing exceptional resolution and light-gathering capability. This objective is optimized for use with oil immersion techniques, which enhance image quality and clarity. The Plan Apo design ensures accurate, distortion-free image reproduction across the field of view.

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Lab products found in correlation

C2 scanning confocal module, by Nikon (1 mentions) Ti e inverted microscope, by Nikon (1 mentions) Nis element, by Nikon (1 mentions) Metamorph 7, by Molecular Devices (1 mentions) Mitotracker green fm, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

60x/1.40 NA Plan Apo oil immersion objective 100× 1.45 NA oil immersion objective Plan Apo 100x oil objective 100x/NA 1.45 oil objective 100×/1.49 NA oil-immersion objective 100x NA 1.45 objective Plan Apo 100x/ 60× oil immersion objective Plan Apo objective Plan Apo I

2 protocols using plan apo 100x na 1.45 oil immersion objective

Confocal Imaging of Biological Samples

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Confocal images were acquired using a Nikon Ti-E inverted microscope equipped with a Nikon C2 scanning confocal module, a Nikon Plan Apo 100X NA 1.45 oil immersion objective and filter cubes. Images were captured with Nikon NIS-Elements software package.

Schiaffarino O., Valdivieso González D., García-Pérez I.M., Peñalva D.A., Almendro-Vedia V.G., Natale P, & López-Montero I. (2022). Mitochondrial membrane models built from native lipid extracts: Interfacial and transport properties. Frontiers in Molecular Biosciences, 9, 910936.

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Quantitative Analysis of Mitochondrial Network

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To assess the mitochondrial network, cells were incubated for 20 min with MitoTracker green FM (Invitrogen™, Carlsbad, CA, USA) and 5 min 0.1 ug/mL Hoechst 33342 to visualise the mitochondria (green) and nucleus (blue). Coverslips were analysed using a microscope ECLIPSE Ti-E equipped with a Plan Apo100x N.A. 1.45 oil immersion objective (Nikon, Tokyo, Japan).). To analyse the mitochondrial network, images acquired by using an Andor NEO sCOMS camera controlled by Metamorph 7.7 software (Molecular Devices) were deconvolved (Huygens Essential, 14.06 version Scientific Volume Imaging Hilversum, The Netherlands) and quantified (Imaris 8.0 ^® software (Oxford Instruments Brand Bitplane AG, Zurich, Switzerland).

Belal S., Goudenège D., Bocca C., Dumont F., Chao De La Barca J.M., Desquiret-Dumas V., Gueguen N., Geffroy G., Benyahia R., Kane S., Khiati S., Bris C., Aranyi T., Stockholm D., Inisan A., Renaud A., Barth M., Simard G., Reynier P., Letournel F., Lenaers G., Bonneau D., Chevrollier A, & Procaccio V. (2022). Glutamate-Induced Deregulation of Krebs Cycle in Mitochondrial Encephalopathy Lactic Acidosis Syndrome Stroke-Like Episodes (MELAS) Syndrome Is Alleviated by Ketone Body Exposure. Biomedicines, 10(7), 1665.

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