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Sw480

Manufactured by Corning
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The SW480 is a laboratory equipment product manufactured by Corning. It is designed for general laboratory use, with the core function of providing a controlled environment for various experimental and testing procedures. The SW480 is a versatile and reliable piece of equipment, but a more detailed description cannot be provided while maintaining an unbiased and factual approach.

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Lab products found in correlation

Sw480, by American Type Culture Collection (9 mentions) Hct116, by Corning (8 mentions) Sw620, by Corning (6 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (5 mentions) Dulbecco modified eagle medium (dmem), by Corning (4 mentions) Hct116, by American Type Culture Collection (3 mentions) Caco 2, by Corning (3 mentions) Rpmi 1640, by Corning (3 mentions) Penicillin streptomycin, by Thermo Fisher Scientific (3 mentions) Penicillin streptomycin, by Corning (2 mentions) Ccd 18co, by Corning (2 mentions) Mycoalert mycoplasma detection kit, by Lonza (2 mentions) Dld 1, by Corning (2 mentions) Colo201, by Corning (2 mentions) Ccd 112con, by Corning (2 mentions) Ccd 33co, by Corning (2 mentions) Caco 2, by American Type Culture Collection (2 mentions) Sw1116, by American Type Culture Collection (2 mentions) Sw1116, by Corning (2 mentions) Mda mb 468, by American Type Culture Collection (2 mentions)

16 protocols using sw480

1

Culturing a CRC cell line panel

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A panel of CRC cell lines was purchased (ATCC) and grown in the vendor-recommended media: DLD-1 (RPMI-1640, Cellgro); HT29 (MacCoy’s 5a modified with 2% NaHCO3, Cellgro); HCT116 (MacCoy’s 5a, Cellgro); Colo201 (RPMI-1640 modified with 1% sodium pyruvate, Cellgro); SW480 and SW620 (Dulbecco’s modified essential medium, Cellgro); CCD-18Co (Eagle’s minimum essential medium, Cellgro); CCD-112CoN (Eagle’s minimum essential medium, Cellgro); CCD-33Co (Eagle’s minimum essential medium, Cellgro). All media were supplemented with 10% FBS and penicillin-streptomycin (Cellgro). All cell lines were tested and determined to be free of mycoplasma contamination (MycoAlert Mycoplasma Detection Kit, Lonza, LT07-418).
Park J., Park J.S., Huang C.H., Jo A., Cook K., Wang R., Lin H.Y., Deun J.V., Li H., Min J., Wang L., Yoon G., Carter B.S., Balaj L., Choi G.S., Castro C.M., Weissleder R, & Lee H. (2021). A high-throughput magneto-electrochemical array for the integrated isolation and profiling of extracellular vesicles from plasma. Nature biomedical engineering, 5(7), 678-689.
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2

Colon Cancer Cell Lines and Culture Conditions

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Colon cancer cell lines LOVO, HCT116, Caco-2, HT29, SW480, and SW1116 were obtained from American Type Culture Collection (ATCC) and subcultured and preserved by Shanghai Institute of Digestive Surgery; normal human colon mucosal epithelium cell NCM460 was kindly donated by Jingqing Zeng (Ruijin Hospital, Shanghai Jiao Tong University School of Medicine; the donator bought NCM460 cell line from INCELL Corporation, LLC, San Antonio, TX, USA). LOVO was cultured in F-12K Nutrient Mixture Medium (Corning Cellgro®, MA, USA), HCT116 and HT29 in McCoy's 5A (Corning Cellgro®), SW480 and SW1116 in Leibovitz' L-15 (Corning Cellgro®), and NCM460 in DMEM (Corning Cellgro®). All culture medium above was supplemented with 10% fetal bovine serum (FBS) (Invitrogen, Carlsbad, CA, USA). Caco-2 was cultured in Minimum Essential Medium (Corning Cellgro®) with 20% FBS. Cells were placed in the incubator (Heracelles, Germany) at 37°C, in a humidified atmosphere with 5% CO2.
Huang A., Zhao H., Quan Y., Jin R., Feng B, & Zheng M. (2014). E2A Predicts Prognosis of Colorectal Cancer Patients and Regulates Cancer Cell Growth by Targeting miR-320a. PLoS ONE, 9(1), e85201.
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3

Cell Line Generation and Culture Protocols

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Mouse embryonic fibroblasts (MEFs) for the indicated genotypes were generated as described previously9 (link). Human lung adenocarcinoma cells (H1299), colorectal adenocarcinoma cells (SW-480) and breast adenocarcinoma cells (MDA-MB-468) were purchased from ATCC and cutaneous SCC cell lines (SRB12, COLO16)30 (link) were a gift from Dr. K. Y. Tsai. The MEF’s, SW-480 and MDA-MB-468 cells were cultured in DMEM (Cellgro) and H1299 cell lines were cultured in RPMI 1640 (Cellgro). The SRB12 and COLO16 cell lines were grown in DMEM/Ham’s F12 50/50 (Cellgro). All cell lines used in the study tested negative for mycoplasma.
Venkatanarayan A., Raulji P., Norton W., Chakravarti D., Coarfa C., Su X., Sandur S.K., Ramirez M.S., Lee J., Kingsley C.V., Sananikone E.F., Rajapakshe K., Naff K., Parker-Thornburg J., Bankson J.A., Tsai K.Y., Gunaratne P.H, & Flores E.R. (2014). IAPP driven metabolic reprogramming induces regression of p53 - deficient tumours in vivo. Nature, 517(7536), 626-630.
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4

Cell Line Generation and Culture Protocols

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Mouse embryonic fibroblasts (MEFs) for the indicated genotypes were generated as described previously9 (link). Human lung adenocarcinoma cells (H1299), colorectal adenocarcinoma cells (SW-480) and breast adenocarcinoma cells (MDA-MB-468) were purchased from ATCC and cutaneous SCC cell lines (SRB12, COLO16)30 (link) were a gift from Dr. K. Y. Tsai. The MEF’s, SW-480 and MDA-MB-468 cells were cultured in DMEM (Cellgro) and H1299 cell lines were cultured in RPMI 1640 (Cellgro). The SRB12 and COLO16 cell lines were grown in DMEM/Ham’s F12 50/50 (Cellgro). All cell lines used in the study tested negative for mycoplasma.
Venkatanarayan A., Raulji P., Norton W., Chakravarti D., Coarfa C., Su X., Sandur S.K., Ramirez M.S., Lee J., Kingsley C.V., Sananikone E.F., Rajapakshe K., Naff K., Parker-Thornburg J., Bankson J.A., Tsai K.Y., Gunaratne P.H, & Flores E.R. (2014). IAPP driven metabolic reprogramming induces regression of p53 - deficient tumours in vivo. Nature, 517(7536), 626-630.
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5

Culturing a CRC cell line panel

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A panel of CRC cell lines was purchased (ATCC) and grown in the vendor-recommended media: DLD-1 (RPMI-1640, Cellgro); HT29 (MacCoy’s 5a modified with 2% NaHCO3, Cellgro); HCT116 (MacCoy’s 5a, Cellgro); Colo201 (RPMI-1640 modified with 1% sodium pyruvate, Cellgro); SW480 and SW620 (Dulbecco’s modified essential medium, Cellgro); CCD-18Co (Eagle’s minimum essential medium, Cellgro); CCD-112CoN (Eagle’s minimum essential medium, Cellgro); CCD-33Co (Eagle’s minimum essential medium, Cellgro). All media were supplemented with 10% FBS and penicillin-streptomycin (Cellgro). All cell lines were tested and determined to be free of mycoplasma contamination (MycoAlert Mycoplasma Detection Kit, Lonza, LT07-418).
Park J., Park J.S., Huang C.H., Jo A., Cook K., Wang R., Lin H.Y., Deun J.V., Li H., Min J., Wang L., Yoon G., Carter B.S., Balaj L., Choi G.S., Castro C.M., Weissleder R, & Lee H. (2021). A high-throughput magneto-electrochemical array for the integrated isolation and profiling of extracellular vesicles from plasma. Nature biomedical engineering, 5(7), 678-689.
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6

Culturing Colorectal Cancer Cell Lines

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Human colorectal cancer cell lines, SW480 and SW1116, were purchased from American Type Culture Collection (ATCC). SW480 and SW1116 were cultured in Leibovitz’ L-15 Medium (Corning Cellgro®, Manassas, VA, USA) with 10% fetal bovine serum (FBS) (Invitrogen, Carlsbad, CA, USA) and 2 mM l-glutamine. Cells were maintained at 37°C/5% CO2 in a humidified incubator.
Liu M., Xu A., Yuan X., Zhang Q., Fang T., Wang W, & Li C. (2015). Downregulation of microRNA-409-3p promotes aggressiveness and metastasis in colorectal cancer: an indication for personalized medicine. Journal of Translational Medicine, 13, 195.
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7

CRC Cell Lines Wnt3a and CGP049090 Treatment

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Human CRC cell lines, SW480 and Caco-2, were purchased from the American Type Culture Collection (Manassas, VA, USA). SW480 was cultured in Leibovitz’s L-15 Medium (Corning Cellgro®, Manassas, VA, USA) and Caco-2 in MEM Medium (Corning Cellgro®). All culture media were supplemented with 10% fetal bovine serum (Invitrogen, Carlsbad, CA, USA). Cells were maintained at 37 °C/5% CO2 in a humidified incubator. Recombinant human Wnt3a (R&D System, Minneapolis, MN, USA) was used at a concentration of 100 ng/mL for treating Caco-2/E12 and Caco-2/E47 cells to activate β-catenin. CGP049090 (Sigma-Aldrich, Lyon, France), a small-molecule inhibitor of Wnt/β-catenin, was diluted in 10 μM for treating SW480/shE2A cells.
Zhao H., Zhao C., Li H., Zhang D, & Liu G. (2019). E2A attenuates tumor-initiating capacity of colorectal cancer cells via the Wnt/beta-catenin pathway. Journal of Experimental & Clinical Cancer Research : CR, 38, 276.
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8

Colorectal and Kidney Cell Lines

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The human colorectal cancer cell line HCT116, SW480, DLD1 and LOVO, as well as the human embryonic kidney cell lines HEK-293T were purchased from ATCC. All cells were grown in RPMI (Corning, 10–040-CV) supplemented with 10% fetal bovine serum (Bioexpress, S1200–500), and penicillin and streptomycin (Lonza, 17–602E). The doxycycline-inducible pINDUCER HCT116, SW480, DLD1, and LOVO cell lines were maintained in the same condition with tetracycline-free fetal bovine serum (Corning, #35–075-CV) and periodically selected by supplemental 500 μg/mL G418 (Sigma, #A1720).
Chen M.S., Lo Y.H., Chen X., Williams C.S., Donnelly J.M., Criss ZK I.I., Patel S., Butkus J.M., Dubrulle J., Finegold M.J, & Shroyer N.F. (2019). Growth Factor–Independent 1 Is a Tumor Suppressor Gene in Colorectal Cancer. Molecular cancer research : MCR, 17(3), 697-708.
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9

Culturing Colorectal Cancer Cell Lines

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Human colorectal cancer cell lines HCT116, HT29, LoVo, SW480, SW620, COLO201, and COLO205 were purchased from the Korean Cell Line Bank (KCLB). HCT116 cells were grown in DMEM medium (Corning, USA), and HT29, LoVo, SW480, SW620, COLO201, COLO205 and LoVo cells were grown in RPMI-1640 medium (Corning, USA) supplemented with 10% Fetal Bovine Serum (Corning, USA), 1% MEM essential amino acids (Corning, USA) and 1% penicillin/streptomycin (Gibco, USA) at 37°C in a humidified atmosphere containing 5% CO2.
Sari N.I., Yang Y.G., Phi L.T., Kim H., Baek M.J., Jeong D, & Kwon H.Y. (2016). Interferon-induced transmembrane protein 1 (IFITM1) is required for the progression of colorectal cancer. Oncotarget, 7(52), 86039-86050.
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10

CRC Cell Line Cultivation Protocol

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The CRC cell lines HT29, Caco2, SW480, SW620, RKO, Lovo, and DLD1 were obtained from the Cell Bank of the Chinese Academy of Sciences (Shanghai, People’s Republic of China), and HCT116 was obtained from KeyGen Biotech (Nanjing, People’s Republic of China). The HCT116 and HT29 cells were maintained in McCoy’s 5A medium (KeyGen Biotech); Dulbecco’s Modified Eagle’s Medium (DMEM) (HyClone, Logan, UT, USA) was used for Lovo, SW480, SW620, RKO, and Caco2, whereas DLD1 cell line was maintained in RPMI-1640 (Corning, Manassas, VA, USA). The media were supplemented with 10% fetal bovine serum (FBS; Gibco BRL, Grand Island, NY, USA) in an incubator with 5% CO2 at 37°C.
Qin C., Ren L., Ji M., Lv S., Wei Y., Zhu D., Lin Q., Xu P., Chang W, & Xu J. (2017). CDKL1 promotes tumor proliferation and invasion in colorectal cancer. OncoTargets and therapy, 10, 1613-1624.
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