A006 1 1

Manufactured by Nanjing Jiancheng

Sourced in China

The A006-1-1 is a laboratory equipment designed for scientific research and analysis. It is a compact and versatile instrument that serves as a general-purpose measurement and testing tool. The core function of the A006-1-1 is to provide accurate and reliable data collection and analysis capabilities for various laboratory applications.

Automatically generated - may contain errors

Lab products found in correlation

Spelling variants of this product

A006-2-1 (39 citations) A006-1 (9 citations) GSH (A006-2-1), (5 citations) GSH (A006-1-1) (3 citations)

11 protocols using a006 1 1

Intracellular Glutathione Quantification

Check if the same lab product or an alternative is used in the 5 most similar protocols

Oxidative stress was confirmed by measuring intracellular GSH levels immediately after exposure to CRA (LD₅₀ = 0.71 μM), as well as in the non-exposed control, using a GSH detection kit (A006-1-1, Jiancheng Bioengineering Institute, Nanjing, Jiangsu, China) as per the manufacturer’s instructions. Briefly, HAECs (1 × 10⁶) were mixed with 1 mM GSH and reagent 1 after CRA exposure, and incubated for 5 min at 37 °C. Thereafter, a mixture of reagents 2–5 was added to the samples and the mixture was incubated at 37 °C for 15 min, before results were detected using an absorbance microplate reader at a wavelength of 420 nm (SpectraMax^® iD3, Molecular Devices, LLC., San Jose, CA, USA).

Xie M.Z., Liu J.L., Gao Q.Z., Bo D.Y., Wang L., Zhou X.C., Zhao M.M., Zhang Y.C., Zhang Y.J., Zhao G.A, & Jiao L.Y. (2022). Proteomics-based evaluation of the mechanism underlying vascular injury via DNA interstrand crosslinks, glutathione perturbation, mitogen-activated protein kinase, and Wnt and ErbB signaling pathways induced by crotonaldehyde. Clinical Proteomics, 19, 33.

+ Open protocol

+ Expand

Placental Oxidative Stress Markers

Check if the same lab product or an alternative is used in the 5 most similar protocols

Kits were used to measure the levels of GSH (Cat. A006-1-1; Nanjing Jiancheng Bioengineering Institute, Nanjing, China), MDA (Cat. S0131S; Beyotime Biotechnology, Shanghai, China), and iron (Cat. E1042-100; Applygen Technology, Beijing, China) in placental tissues and cells according to the manufacturer’s instructions. Briefly, after the lysis of tissue samples or cells, the supernatant was collected after centrifugation at 10,000–12,000 g for 10 min at 4 °C. The content was measured on a colorimetric microplate reader (Thermo Scientific, Multiskan, FC, USA). Protein concentrations were measured using the kit according to the manufacturer’s instructions (Cat. P0010; Beyotime Biotechnology, Shanghai, China). Then, the GSH, MDA and iron values were homogenized by protein concentration.

Yang H., Zhang X., Ding Y., Xiong H., Xiang S., Wang Y., Li H., Liu Z., He J., Tao Y., Yang H, & Qi H. (2022). Elabela: Negative Regulation of Ferroptosis in Trophoblasts via the Ferritinophagy Pathway Implicated in the Pathogenesis of Preeclampsia. Cells, 12(1), 99.

+ Open protocol

+ Expand

Glutathione Quantification Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols

The GSH levels in the plasma, tissue homogenate, and cell extracts were performed according to the manufacturer's protocols (A006‐1‐1; Nanjing Jiancheng, China). Glutathione levels were detected by using the DTNB‐GSSG reductase recycling methods. A405 was determined by enzyme marker.

Shi X., Liu R., Wang Y., Yu T., Zhang K., Zhang C., Gu Y., Zhang L., Wu J., Wang Q, & Zhu F. (2024). Inhibiting acid‐sensing ion channel exerts neuroprotective effects in experimental epilepsy via suppressing ferroptosis. CNS Neuroscience & Therapeutics, 30(2), e14596.

+ Open protocol

+ Expand

Quantitative Evaluation of Myocardial Oxidative Markers

Check if the same lab product or an alternative is used in the 5 most similar protocols

The expression levels of MDA (A003-1-2, Nanjing Jiancheng, Nanjing, China), GSH (A006-1-1, Nanjing Jiancheng, Nanjing, China) and CAT (A007-2-1, Nanjing Jiancheng, Nanjing, China) in myocardial tissue homogenate or cell supernatant were detected according to the relevant kit instructions. The intracellular ROS level was determined using the commercial kits (Sigma-Aldrich, St. Louis, MO, USA) based on the turn out of the 2’7’-dichlorofluorescin diacetate (DCF-DA) into highly fluorescent 2’,7’-dichlorofluorescein (DCF) in line with the manufacturer’s instructions. The fluorescence intensity was measured by a fluorescence microscopy (Olympus, Tokyo, Japan).

Zhou L., Zhai G, & Tian G. (2024). CRIF1 attenuates doxorubicin-mediated mitochondrial dysfunction and myocardial senescence via regulating PXDN. Aging (Albany NY), 16(6), 5567-5580.

+ Open protocol

+ Expand

Plasma Immunological and Antioxidant Markers

Check if the same lab product or an alternative is used in the 5 most similar protocols

Plasma immunoglobulin M (IgM), complement 3 (C3), complement 4 (C4) contents and lysozyme (LZM) activity were determined using the immunoprotein assay kit (H109-1-1, H186-1-1, H186-2-1, A150-1-1; Jiancheng Bioengineering Institute, Nanjing, China) by enzyme linked immunosorbent assay (ELISA) according to the manufacturer’s instructions. The contents of IgM, C3 and C4 were calculated by the standard curve method and LZM activity was determined using the turbidimetric method. The activities of total antioxidant capacity (T-AOC) and superoxide dismutase (SOD), glutathione (GSH), catalase (CAT) and content of malondialdehyde (MDA) in plasma were determined by using the kits (A105-1-1, A001-1-2, A006-1-1, A007-2-1, A003-1-2; Jiancheng Bioengineering Institute, Nanjing, China). T-AOC and SOD, GSH, CAT and MDA of plasma were determined by the ferric ion reducing antioxidant power (FRAP) method, hydroxylamine method, spectrophotometric method and thiobarbituric acid (TBA) method, respectively.

He C., Geng H., Qin Y., Yang P., Wang W., Mai K, & Song F. (2022). Dietary xanthophyll improved growth, antioxidant, pigmentation and meat quality in the southern catfish (Silurus soldatovi meridionalis Chen). Animal Nutrition, 13, 101-115.

+ Open protocol

+ Expand

Cytokine and Oxidative Stress Evaluation

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cytokines was correlated tightly with the occurrence of the infection, interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α) and interleukin-1 (IL-1), while myeloperoxidase (MPO), malondialdehyde (MDA), glutathione (GSH) and superoxide dismutase (SOD) were correlated with oxidative stress reaction, which was often occurred in UC. So here we measured this cytokinesis to evaluate the efficacy of the treatment. IL-6 (SEA079Ra; Cloud-Clone Corp) and TNF-α (SEA133Ra; Cloud-Clone Corp) were quantified using commercially available ELISA kits. Standard curves are shown in Supplementary Figure S1D,E. MPO (A044-1-1; Nanjing Jiancheng Bioengineering Institute), GSH (A006-1-1; Nanjing Jiancheng Bioengineering Institute), MDA (A003-1-2; Nanjing Jiancheng Bioengineering Institute) and T-SOD (A001-1-2; Nanjing Jiancheng Bioengineering Institute levels in sera were determined by ELISA assay kits according to the manufacturer’s instructions.

Rong Y., Hong G., Zhu N., Liu Y., Jiang Y, & Liu T. (2021). Photodynamic Therapy of Novel Photosensitizer Ameliorates TNBS-Induced Ulcerative Colitis via Inhibition of AOC1. Frontiers in Pharmacology, 12, 746725.

+ Open protocol

+ Expand

Hepatopancreas Biochemical Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

Hepatopancreas were homogenized with PBS (1:9) and centrifuged immediately at 4000 × g, 4°C for 10 min. The obtained supernatants were used to measure biochemical parameters with relevant kits. Specifically, total protein was measured with BCA Protein Assay Kit (A045-3, Jiancheng Bioengineering Institute, Nanjing, China). Total antioxidant capacity (T-AOC), catalase (CAT), superoxide dismutase (SOD), reduced glutathione (GSH), and malondialdehyde (MDA) in supernatants were analyzed with A015–1, A007-1-1, A001–1, A006-1-1, and A003-1 kits (Jiancheng Bioengineering Institute, Nanjing, China), respectively. In addition, total cholesterol (T-CHO), lysozyme (LZM), aspartate aminotransferase (AST), and alanine aminotransferase (ALT) in plasma were detected using A111-1-1, A050-1-1, C010-1-1, and C009-1-1 kits (Jiancheng Bioengineering Institute, Nanjing, China), respectively. The phenoloxidase (PO) determination was as referenced by Lin et al. (2012) (link) with kits (G0146W, Suzhou Grace Biotechnology Co., Ltd., China).

Zheng L., Liu Y., Zhang Y., Xu B., Sagada G., Wang Z., Chen C., Lang X., Zhang J, & Shao Q. (2023). Comparative study on the effects of crystalline L-methionine and methionine hydroxy analogue calcium supplementations in the diet of juvenile Pacific white shrimp (Litopenaeus vannamei). Frontiers in Physiology, 14, 1067354.

+ Open protocol

+ Expand

Histomorphological and Biochemical Analyses of Intestinal Tissues

Check if the same lab product or an alternative is used in the 5 most similar protocols

The duodenum, jejunum, ileum tissues were microscopically examined after fixing in 10% neutral-buffered formalin and processing for paraffin embedding, sectioning at 5 μm, and then staining with hematoxylin and eosin [17 (link)]. The concentrations of lipopolysaccharide (LPS), malondialdehyde (MDA), protein carbonyl (PC) and reduced glutathione (GSH) and activity of diamine oxidase (DAO), superoxide dismutase (SOD), total antioxidant capacity (T-AOC) were measured by a colorimetric method with the use of specific assay kits (H255, A003-1-2, A087-1-2, A006-1-1, A088-1-1, A001-1-2 and A015-1-2) from the Nanjing Jiancheng Bioengineering Institute of China. Protein concentration was measured by the bicinchoninic acid assay (Beyotime Institute of Biotechnology, Jiangsu, China).

Liu M., Zhang L., Mo Y., Li J., Yang J., Wang J., Karrow N.A., Wu H, & Sun L. (2023). Ferroptosis is involved in deoxynivalenol-induced intestinal damage in pigs. Journal of Animal Science and Biotechnology, 14, 29.

+ Open protocol

+ Expand

Cortical Injury Oxidative Stress Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

Injured cortical samples were homogenized with 0.9% NaCl and centrifuged to collect the supernatant according to the manufacturer’s instructions. A glutathione (GSH) detection assay (A006-1-1; NanJing JianCheng Bioengineering Institute, Nanjing, China) was used. The malondialdehyde (MDA) levels were measured according to the instructions of the MDA detection assay (A003-1-2; NanJing JianCheng Bioengineering Institute, Nanjing, China). The amount of GSH or MDA was normalized to the total protein level and expressed as GSH/total protein or MDA/total protein.

Cheng H., Wang N., Ma X., Wang P., Dong W., Chen Z., Wu M., Wang Z., Wang L., Guan D, & Zhao R. (2022). Spatial-temporal changes of iron deposition and iron metabolism after traumatic brain injury in mice. Frontiers in Molecular Neuroscience, 15, 949573.

+ Open protocol

+ Expand

Antioxidant Enzyme Analysis in Mouse Kidneys

Check if the same lab product or an alternative is used in the 5 most similar protocols

Mice renal samples were homogenized and centrifuged. The supernatant was then collected for SOD, CAT, and GSH analysis using kits (A001-1-2, A007-2-1, and A006-1-1, Nanjing Jiancheng Bioengineering Institute) according to the manufacturer’s protocols.

Chen P., Liu W, & Wei Y. (2023). Ramelteon attenuates renal ischemia and reperfusion injury through reducing mitochondrial fission and fusion and inflammation. Translational Andrology and Urology, 12(12), 1859-1870.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!