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Phage gfp

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PHAGE-GFP is a bacterial virus (bacteriophage) that has been genetically modified to express the green fluorescent protein (GFP). This allows for the visualization and tracking of the bacteriophage within a sample.

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Lab products found in correlation

Gateway lr clonase 2 enzyme mix, by Thermo Fisher Scientific (2 mentions) Anti v5 tag antibody, by Merck Group (2 mentions) Anti flag tag antibody, by Merck Group (2 mentions)

Close spelling variants of this product

Phage-ubc-nls-ha-tdMCP-GFP (3 citations) PHAGE-CMV-dsRed-UBC-GFP-W (2 citations)

2 protocols using phage gfp

1

Overexpression of DNMT3A, DNMT3B, and RING1 in GM12878 Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Entry vectors bearing DNMT3A, DNMT3B,
or RING1 cDNAs were purchased from the Harvard Plasmid
Repository. DNMT3A or DNMT3B cDNAs were cloned into pLX-TRC313 (Broad Institute)
using Gateway LR Clonase II Enzyme mix (Thermo Fisher Scientific). Control
vector pLX-TRC313-GFP was obtained from Broad Institute. GM12878 LCLs were
transduced with pLX-TRC313-GFP, -DNMT3A, or -DNMT3B encoding lentiviruses. Cells
were selected with hygromycin for 2 weeks. Heterogenous protein expression was
confirmed by immunoblot using anti-V5 tag antibody (V8012, Sigma-Aldrich).
RING1 cDNA was cloned into pHAGE-3×FLAG-MYC vector
using Gateway LR Clonase II Enzyme mix (Thermo Fisher Scientific). Control
vector pHAGE-GFP was obtained from Addgene (plasmid #106281). GM12878 LCLs were
transduced with either pHAGE-GFP or RING1 encoding lentiviruses. Cells were
selected with puromycin for 4 days. Heterogenous protein expression was
confirmed by immunoblot using anti-Flag tag antibody (F1804, Sigma-Aldrich).
Guo R., Zhang Y., Teng M., Jiang C., Schineller M., Zhao B., Doench J.G., O’Reilly R.J., Cesarman E., Guilino-Roth L, & Gewurz B.E. (2020). DNA Methylation Enzymes and PRC1 Restrict B-cell Epstein-Barr Virus Oncoprotein Expression. Nature microbiology, 5(8), 1051-1063.
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2

Overexpression of DNMT3A, DNMT3B, and RING1 in GM12878 Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Entry vectors bearing DNMT3A, DNMT3B,
or RING1 cDNAs were purchased from the Harvard Plasmid
Repository. DNMT3A or DNMT3B cDNAs were cloned into pLX-TRC313 (Broad Institute)
using Gateway LR Clonase II Enzyme mix (Thermo Fisher Scientific). Control
vector pLX-TRC313-GFP was obtained from Broad Institute. GM12878 LCLs were
transduced with pLX-TRC313-GFP, -DNMT3A, or -DNMT3B encoding lentiviruses. Cells
were selected with hygromycin for 2 weeks. Heterogenous protein expression was
confirmed by immunoblot using anti-V5 tag antibody (V8012, Sigma-Aldrich).
RING1 cDNA was cloned into pHAGE-3×FLAG-MYC vector
using Gateway LR Clonase II Enzyme mix (Thermo Fisher Scientific). Control
vector pHAGE-GFP was obtained from Addgene (plasmid #106281). GM12878 LCLs were
transduced with either pHAGE-GFP or RING1 encoding lentiviruses. Cells were
selected with puromycin for 4 days. Heterogenous protein expression was
confirmed by immunoblot using anti-Flag tag antibody (F1804, Sigma-Aldrich).
Guo R., Zhang Y., Teng M., Jiang C., Schineller M., Zhao B., Doench J.G., O’Reilly R.J., Cesarman E., Guilino-Roth L, & Gewurz B.E. (2020). DNA Methylation Enzymes and PRC1 Restrict B-cell Epstein-Barr Virus Oncoprotein Expression. Nature microbiology, 5(8), 1051-1063.
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