Witepsol E85 (W) was supplied by Cremer Oleo (Hamburg, Germany); Gelucire 44/14 (G) was kindly provided by Gattefossé SAS (Saint-Priest, France). Ethanol, polyvinylalcohol (PVA), dichloromethane (DCM) and octan-1-ol were acquired from Sigma-Aldrich (St. Louis, MO, USA). Acetonitrile and dimethylsulfoxide (DMSO) were acquired from Merck (Darmstadt, Germany). Ultrapure bidistilled water was obtained by a MilliQ R4 system, Millipore (Milan, Italy). The fresh intestine was taken from a local slaughterhouse (Forma s.r.l; CE IT D2L5B (Regulation EC 853/2004)). Fetal bovine serum (FBS), Dulbecco’s modified Eagle’s medium (DMEM/F12, HEPES, no phenol red) and streptomycin–penicillin were acquired from Life Technologies Italia (Monza, Italy). Phosphate-buffered saline (PBS, NaCl 0.138 M; KCl 0.0027 M; pH 7.4, at 25 °C), Tween80 and 3-(4,5-dimethyl-thiazol-2-yl)-2,5,diphenyltetrazoliumbromide (MTT, 97.5%) were obtained from Sigma-Aldrich (Milan, Italy).
Octan 1 ol
Manufactured by Merck Group
Sourced in Germany
Octan-1-ol is a primary alcohol with the chemical formula CH3(CH2)7OH. It is a colorless, oily liquid with a characteristic odor. Octan-1-ol is a common organic compound used in various industrial and laboratory applications.
Automatically generated - may contain errors
Lab products found in correlation
3 4 5 dimethylthiazol 2 yl 2 5 diphenyltetrazolium bromide mtt, by Merck Group (1 mentions)
Milliq r4 system, by Merck Group (1 mentions)
Dichloromethane, by Merck Group (1 mentions)
Polyvinyl alcohol (pva), by Merck Group (1 mentions)
Phosphate buffered saline (pbs), by Merck Group (1 mentions)
Tween 80, by Merck Group (1 mentions)
Acetonitrile, by Merck Group (1 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (1 mentions)
Milli q system, by Merck Group (1 mentions)
Ethanol, by Merck Group (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Trifluoroacetic acid tfa, by Merck Group (1 mentions)
Resazurin sodium salt, by Merck Group (1 mentions)
4 protocols using octan 1 ol
1
Intestinal Permeability and Cytotoxicity Evaluation
2
Preparation of Octan-1-ol and Ethanol Solutions
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Octan-1-ol (≥98%) and ethanol (≥99.8%) were purchased from Sigma–Aldrich and used as received. Ultrapure water (18.2 MΩ) was produced from a Millipore Milli-Q system.
3
Cytotoxicity Evaluation of Organoruthenium Complex
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CDs and N-CDs were prepared and characterized as previously described [22 (link),23 (link)]. Organoruthenium complex, cyano(cyclopentadienyl)bis(triphenylphosphine)ruthenium(II), [Ru(η5-Cp)(PPh3)2CN] (RuCN), was prepared by following a published method [24 (link)].
A2780 ovarian cancer cell line was obtained from the European Collection of Cell Cultures (ECACC, Salisbury, UK). The cell culture medium, fetal bovine serum (FBS), and antibiotics were purchased from Life Technologies (Paisley, UK). Resazurin sodium salt (75.0% of dye content) was bought from Sigma Aldrich and dissolved in PBS (phosphate buffer solution, Sigma Aldrich) to prepare the solution (0.1 mg/mL) for the cytotoxicity assays.
Matrix for MALDI TOF MS (2,5-dihydroxybenzoic acid (DHB), octan-1-ol, methanol, and trifluoroacetic acid (TFA)) was purchased from Sigma-Aldrich (Taufkirchen, Germany) and used without further purification.
A2780 ovarian cancer cell line was obtained from the European Collection of Cell Cultures (ECACC, Salisbury, UK). The cell culture medium, fetal bovine serum (FBS), and antibiotics were purchased from Life Technologies (Paisley, UK). Resazurin sodium salt (75.0% of dye content) was bought from Sigma Aldrich and dissolved in PBS (phosphate buffer solution, Sigma Aldrich) to prepare the solution (0.1 mg/mL) for the cytotoxicity assays.
Matrix for MALDI TOF MS (2,5-dihydroxybenzoic acid (DHB), octan-1-ol, methanol, and trifluoroacetic acid (TFA)) was purchased from Sigma-Aldrich (Taufkirchen, Germany) and used without further purification.
4
Octanol-based Solvent Characterization
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Octan-1-ol (purity >99%, for simplicity
referred
to as “octanol”) was purchased from Sigma-Aldrich Chemie
GmbH (Steinheim, Germany). Ethanol (purity >99.5%) and sulfuric
acid
(96 wt %) were provided by Carlo Erba Reagents (Cornaredo, Italy).
All samples were prepared with deionized water, with a conductivity
of 6.5–12.3 μS·cm–1. The solvents
were used without any further purification steps. The term dry octanol is attributed to noncontacted octanol, without
any drying procedures. The water content from contact with the environment
was found to be 452 ppm and determined by coulometric Karl Fischer
titration.
referred
to as “octanol”) was purchased from Sigma-Aldrich Chemie
GmbH (Steinheim, Germany). Ethanol (purity >99.5%) and sulfuric
acid
(96 wt %) were provided by Carlo Erba Reagents (Cornaredo, Italy).
All samples were prepared with deionized water, with a conductivity
of 6.5–12.3 μS·cm–1. The solvents
were used without any further purification steps. The term dry octanol is attributed to noncontacted octanol, without
any drying procedures. The water content from contact with the environment
was found to be 452 ppm and determined by coulometric Karl Fischer
titration.
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