Anti ki 67 clone b56

Manufactured by BD

Sourced in Germany

Anti-Ki-67 (clone B56) is an immunohistochemical reagent used for the detection of the Ki-67 protein, a cellular marker for proliferation. The clone B56 is a mouse monoclonal antibody that specifically binds to the Ki-67 antigen, which is present in the nuclei of proliferating cells.

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Lab products found in correlation

Anti cd69 clone fn50, by BioLegend (1 mentions) Anti cd154 clone 24 31, by BioLegend (1 mentions) Live dead fixable aqua dead cell stain kit, by Thermo Fisher Scientific (1 mentions) Lsr 2 flow cytometer, by BD (1 mentions) Alexa fluor 488 anti mouse igg, by Thermo Fisher Scientific (1 mentions) Alexa fluor 546 anti rabbit igg, by Thermo Fisher Scientific (1 mentions) Hoechst 33258, by Merck Group (1 mentions) Bz 9000 fluorescence microscope, by Keyence (1 mentions) Fluorsave reagent, by Merck Group (1 mentions) Foxp3 transcription factor staining buffer kit, by Thermo Fisher Scientific (1 mentions) Live dead dead cell stain kit, by Thermo Fisher Scientific (1 mentions) Lsrfortessa cell analyzer, by BD (1 mentions)

Close spelling variants of this product

Mouse anti-human Ki-67 (clone B56; Anti-Ki-67-PE (clone B56; Ki-67

3 protocols using anti ki 67 clone b56

Activation-Induced Marker (AIM) Staining of Antigen-Specific T Cells

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After peptide stimulation, the cells were washed with PBS containing 5% FCS and stained with amine-reactive viability stain (Live/dead fixable aqua dead cell stain kit; Invitrogen) for 30 min at 4°C. After washing, pMHC-spheromers were added to screen the epitope-specific CD8⁺ and CD4⁺ T cells. Meanwhile, the antibody cocktail was added for AIM staining (anti-CD69 (clone FN50, Biolegend), anti-CD154 (clone 24-31, Biolegend), anti-CD137 (clone 4B4-1, Biolegend), anti-CD38 (clone HIT2, BD Biosciences) and anti-Ki-67 (clone B56, BD Biosciences)). The cells were stained for 30 min at 4°C in 100μl volume.
Cells were then washed twice with staining buffer before acquisition using BD LSRII flow cytometer. The data was analyzed using FlowJo (v10) software.

Gao F., Mallajoysula V., Arunachalam P.S., van der Ploeg K., Manohar M., Röltgen K., Yang F., Wirz O., Hoh R., Haraguchi E., Lee J.Y., Willis R., Ramachandiran V., Li J., Kathuria K.R., Li C., Lee A.S., Shah M.M., Sindher S.B., Gonzalez J., Altman J.D., Wang T.T., Boyd S.D., Pulendran B., Jagannathan P., Nadeau K.C, & Davis M.M. (2023). Spheromers reveal robust T cell responses to the Pfizer/BioNTech vaccine and attenuated peripheral CD8+ T cell responses post SARS-CoV-2 infection. Immunity, 56(4), 864-878.e4.

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Immunofluorescence Analysis of SDHB and Ki67

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Cells were seeded on cover slips (ø 18 mm; Thermo Scientific) and cultured as described. Immunofluorescence staining was performed as previously described [39 (link)]. The following antibodies were used: 1.7 µg/mL anti-SDHB (clone EPR10880, Abcam), 10 µg/mL anti-Ki67 clone B56; BD Biosciences, Heidelberg, Germany) and 4 µg/mL of either anti-mouse IgG-Alexa Fluor 488 or anti-rabbit IgG-Alexa Fluor546 (Thermo Scientific). Nuclear staining was performed by incubation in Hoechst 33258 (Sigma-Aldrich, St. Louis, MO, USA) diluted at 2 µg/mL in PBS for 1 hour at RT. Afterwards, samples were mounted in FluorSave Reagent (Merck Millipore, Darmstadt, Germany) on microscope slides. Staining specificity was verified by staining with isotype control antibodies (rabbit IgG, clone SP137, Abcam). Images were taken with a Keyence BZ-9000 fluorescence microscope and relative quantification was performed making use of the Hybrid Cell Count tool provided by BZ-9000 Image Analysis Application (both from Keyence, Neu-Isenburg, Germany).

Rahn S., Dänzer Barbosa P., Möller J.L., Ammar N., Demetrowitsch T., Helm O., Wesch D., Sipos B., Röcken C., Schwarz K., Schäfer H, & Sebens S. (2019). Inflammation Associated Pancreatic Tumorigenesis: Upregulation of Succinate Dehydrogenase (Subunit B) Reduces Cell Growth of Pancreatic Ductal Epithelial Cells. Cancers, 12(1), 42.

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Multi-Omics Analysis of Lymph Node Immune Cells

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Multi-color flow cytometric analysis was performed on mononuclear cells isolated from iliac LN samples. The following antibodies were used: LIVE/DEAD dead cell stain kit (Invitrogen); anti-CD8a (clone RPA-T8), anti-CD4 (clone OKT4), anti-PD-1 (clone EH12.2H7), anti-ICOS (clone C398.4A), anti-CD25 (clone BC96), anti-CXCR3 (clone G025H7) (BioLegend); anti-CXCR5 (clone MU5UBEE), anti-FOXP3 (eBioscience); anti-Bcl-6 (clone K112-91), anti-CD95 (clone DX2), anti-CD3 (clone SP34-2), and anti-Ki-67 (clone B56) (BD Biosciences); and anti-CD20 (clone B9E9), IgG (clone G18-145), IgM (G20-127) (Beckman Coulter).
For each BG505 SOSIPv5.2 Env trimer probe analysis, the biotinylated probes were individually premixed with fluorochrome-streptavidin conjugates (SA-Alexa647 and SA-BV421, Thermo Fisher Scientific and BioLegend) at room temperature (RT) for 20 min. After surface staining followed by washes, cells were fixed and permeabilized using FoxP3/Transcription Factor Staining Buffer kit (Thermo Fisher Scientific) according to manufacturer's protocols. Upon permeabilization, cells were stained with intranuclear Abs, washed twice and acquired on an LSR Fortessa Cell Analyzer (BD Biosciences). Flow cytometry data were analyzed with FlowJo (Tree Star).

Carnathan D.G., Kaushik K., Ellebedy A.H., Enemuo C.A., Gebru E.H., Dhadvai P., Rasheed M.A., Pauthner M.G., Ozorowski G., Ahmed R., Burton D.R., Ward A.B., Silvestri G., Crotty S, & Locci M. (2020). Harnessing Activin A Adjuvanticity to Promote Antibody Responses to BG505 HIV Envelope Trimers. Frontiers in Immunology, 11, 1213.

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