Digoxin (D6003), ouabain (O3125), McCoy’s 5A medium (M4892), and anti-α-tubulin antibody (T6074) were purchased from Sigma. Bufalin (025-15241) was obtained from Wako Pure Chemical Industries. LY294002 (9901), anti-Aurora A/AIK (1G4) (4718), Anti-phospho-Aurora A (Thr288)/Aurora B (Thr232) (2914), anti-phospho-Aurora A (Thr 288) (3079), and anti-histone H3 (9715) were obtained from Cell Signaling Technology. The anti-histone H3 (phospho S10) (ab14955) antibody was from Abcam. Anti-Aurora B antibody (1788-1) was from Epitomics Inc. Anti-Aurora B pT232 (600-401-677) and anti-α-Tubulin (600-301-880) antibodies were obtained from Rockland immunochemicals. Alexa Fluor 488 Donkey anti-Rabbit lgG (A21206) and Alexa Fluor 555 Donkey anti-Mouse lgG (A31570) antibodies were purchased from Life Technologies Corporation. PI (propidium iodide)/RNase staining buffer solution (550825) was purchased from BD Pharmingen. Fetal bovine serum (FBS; 16000-044) and Dulbecco’s Modified Eagle medium (DMEM; 12800-017) were gotten from Gibco Invitrogen. Anti-mouse lgG-HRP (sc-2005) and anti-rabbit lgG-HRP (sc-2004) antibodies were obtained from Santa Cruz Biotechnology. Chemiluminescence HRP Substrate (WBKLS0500) antibody was from Millipore.
Anti rabbit lgg hrp sc 2004
Manufactured by Santa Cruz Biotechnology
Anti-rabbit IgG-HRP (sc-2004) is a secondary antibody conjugated with horseradish peroxidase (HRP). It is used to detect and visualize rabbit primary antibodies in various immunoassays.
Automatically generated - may contain errors
Lab products found in correlation
Anti α tubulin antibody t6074, by Merck Group (1 mentions)
Anti histone h3, by Cell Signaling Technology (1 mentions)
Anti phospho ulk1 ser757, by Cell Signaling Technology (1 mentions)
Anti atg5, by Cell Signaling Technology (1 mentions)
Anti phospho mtor ser2448, by Cell Signaling Technology (1 mentions)
Anti lc3b antibody l7543, by Merck Group (1 mentions)
Anti ulk1 d8h5, by Cell Signaling Technology (1 mentions)
2 protocols using anti rabbit lgg hrp sc 2004
1
Comprehensive Molecular Analysis of Aurora Kinase
2
Autophagy Signaling Pathway Assays
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Alexa Fluor 488 donkey anti-rabbit lgG (A21206) antibody was purchased from Life Technologies Corporation. Fetal bovine serum (16000-044) was purchased from Gibco Invitrogen. McCoy’s 5A medium (M4892), 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT; M2128), cycloheximide (C7698), MG132 (M7449), pepstatin A (P5318) and anti-LC3B antibody (L7543) were obtained from Sigma. Anti-BECN1 (sc-10086), anti-SQSTM1/p62 (H-290) (sc-25575), anti-mouse lgG-HRP (sc-2005) and anti-rabbit lgG-HRP (sc-2004) antibodies were obtained from Santa Cruz Biotechnology. Chemiluminescence HRP substrate (WBKLS0500) was purchased from Millipore. Anti-AMPKα (#2532S), anti-phospho-AMPKα (Thr172) (#2535), anti-phospho-ULK1 (Ser757) (#6888), anti-phospho-MTOR (Ser2448) (#2971), anti-ATG5 (#2630), anti-ATG7 (#2631), anti-EEF2 (#2332), anti-phospho-EEF2 (Thr56) (#2331), anti-ULK1 (D8H5) (#8054), anti-phospho-ULK1 (Ser555) (D1H4) (#5869) and anti-EEF2K (#3692) antibodies were obtained from Cell Signaling Technology.
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