The miR-7 mimic/inhibitor, pcDNA3.1-cdc42, si-cdc42, and their respective control were chemically synthesized or purchased from GenePharma Co. (Shanghai, China) and transfected (100 nM) or co-transfected into NSCs cells using Invitrogen Lipofectamine™ 2000 transfection reagent (Thermo Fisher Scientific). The detailed information regarding miR-7 mimic and miR-7 inhibitor and their controls is listed as follows: miR-7 mimic: (sense: 5′ -UGGAAGACUAGUGAUUUUGUUGU-3′, antisense: 5′ -AACAAAAUCACUAGUCUUCCAUU-3′); the negative control of miR-7 mimic (pre-NC, sense: 5′ -UUCUCCGAACGUGUCACGUTT-3′, antisense: 5′ -ACGUGACACGUUCGGAGAATT-3′); miR-7 inhibitor: (5′ -ACAACAAAAUCACUAGUCUUCCA- 3′), the negative control of miR-7 inhibitor (NC, 5′ -CAGUACUUUUGUG UAGUACA A-3′).
Si cdc42
Manufactured by GenePharma
Sourced in China
Si-cdc42 is a lab equipment product that serves as a small GTPase. It is a member of the Rho family of proteins and plays a fundamental role in various cellular processes, including cytoskeleton organization, cell signaling, and cell cycle regulation.
Automatically generated - may contain errors
Lab products found in correlation
Invitrogen lipofectamine 2000 transfection reagent, by Thermo Fisher Scientific (1 mentions)
Lipofectamine rnaimax, by Thermo Fisher Scientific (1 mentions)
Actingreen 488, by Thermo Fisher Scientific (1 mentions)
Anti collagen 1, by Abcam (1 mentions)
Fm4 64fx, by Thermo Fisher Scientific (1 mentions)
Anti rac1, by Cell Signaling Technology (1 mentions)
Anti cd34, by Abcam (1 mentions)
Anti alix, by Abcam (1 mentions)
Anti fibronectin, by Abcam (1 mentions)
Alexa fluor 488, by Proteintech (1 mentions)
Anti β actin, by Cell Signaling Technology (1 mentions)
Anti rhoa, by Cell Signaling Technology (1 mentions)
Anti cdc42, by Cell Signaling Technology (1 mentions)
Anti cd90, by Abcam (1 mentions)
Ml141, by MedChemExpress (1 mentions)
Anti cd45, by Abcam (1 mentions)
Anti ki67, by Cell Signaling Technology (1 mentions)
Anti cd105, by Abcam (1 mentions)
Anti cd73, by Abcam (1 mentions)
Anti cd63, by Abcam (1 mentions)
2 protocols using si cdc42
1
miR-7 Modulation in Neural Stem Cells
2
Characterization of Mesenchymal Stem Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Anti-CD9, anti-CD63, anti-Alix, anti-calnexin, anti-CD31, anti-CD34, anti-CD45, anti-CD73, Anti-CD90, anti-CD105, anti-Collagen I, and anti-Fibronectin antibodies were purchased from Abcam (Cambridge, UK). Anti-Ki-67, anti-RhoA, anti-Rac1, anti-Cdc42, and anti-β-actin antibodies were purchased from Cell Signaling Technology (Danvers, USA). Alexa Fluor 488- and Alexa Fluor 568-conjugated secondary antibodies were purchased from Proteintech (Rosemont, IL, USA). IRDye 800cw-conjugated goat anti-rabbit/anti-mouse IgG secondary antibodies were purchased from Abbkine (Redlands, CA, USA). PKH-26 and PKH-67 kits were purchased from Sigma-Aldrich (St. Louis, MO, USA). Lipofectamine™ RNAiMAX, FM™ 4-64FX, and ActinGreen™ 488 were purchased from Thermo Fisher (Eugene, Oregon, USA). SiCdc42, Cdc42-EGFP, and Cdc42-mCherry fusion protein expression plasmids were purchased from GenePharma (Suzhou, China). The Cdc42 inhibitor ML141 was purchased from MedChemExpress (Monmouth Junction, USA).
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