All chemicals used for caffeine analysis were of analytical grade. Bulk solvents and routine chemicals were procured from SISCO research laboratory (Mumbai, India). Caffeine standard and diphenylamine were procured from Sigma Aldrich, USA.
Caffeine standard
Manufactured by Merck Group
Sourced in United States
Caffeine standard is a laboratory reference material used for the identification and quantification of caffeine in various samples. It provides a consistent and reliable source of pure caffeine for analytical purposes.
Automatically generated - may contain errors
4 protocols using caffeine standard
1
Analytical Caffeine Extraction Protocol
2
Optimized Caffeine Extraction and HPLC Analysis
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The Kromasil Ethernity C18 column (2.1 × 50 mm) with a particle size of 2.5 μm was applied (Nouryon AB, Bohus, Sweden). Water was purified with a Milli-Q Water Purification System (Millipore Corporation, Bedford, MA, USA). HPLC-grade ethanol was obtained from Sigma-Aldrich (Saint Luis, MO, USA). The caffeine standard was purchased from Sigma-Aldrich (Saint Luis, MO, USA). Polymeric SPE cartridges Strata™-X 33 μm (200 mg/3 mL) were purchased from Phenomenex (Torrance, CA, USA).
3
Caffeine Extraction from Arabica Coffee
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Caffeine standard (99.8%) was obtained from Sigma Aldrich (India). All reagents and solvents were analytical and HPLC grade. The bean material of C. arabica was procured from Sargod Trading Co., Chikmagalur, Karnataka, India.
4
Evaluation of 5-Chloroquinic Acid Purity
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Three commercial standards of 5-chloroquinic acid (5-CQA) were evaluated for purity: Sigma-Aldrich (USA), Chromadex (USA), and Phytolab (Germany). Caffeine standard was purchased from Sigma. Methanol and acetonitrile were high-pressure liquid chromatography (HPLC) grade and obtained from Fisher Chemical. Formic acid was reagent grade (Sigma) and was mixed at the appropriate concentration in water. Water was purified from a Millipore Direct Q UV3 system.
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